PCNA (Ab-1) Mouse mAb (PC10) | EMD Millipore NA03-200UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PCNA (Ab-1) Mouse mAb (PC10)

catalog :

NA03-200UG

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig 2a	Mescher M, Jeong P, Knapp S, Rübsam M, Saynisch M, Kranen M, et al. The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma. J Exp Med. 2017;214:339-358 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3b	Gago Fuentes R, Bechberger J, Varela Eirin M, Varela Vazquez A, Acea B, Fonseca E, et al. The C-terminal domain of connexin43 modulates cartilage structure via chondrocyte phenotypic changes. Oncotarget. 2016;7:73055-73067 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 5	Yamamoto T, Hasegawa T, Sasaki M, Hongo H, Tsuboi K, Shimizu T, et al. Frequency of Teriparatide Administration Affects the Histological Pattern of Bone Formation in Young Adult Male Mice. Endocrinology. 2016;157:2604-20 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 2	Camlin N, Sobinoff A, Sutherland J, Beckett E, Jarnicki A, Vanders R, et al. Maternal Smoke Exposure Impairs the Long-Term Fertility of Female Offspring in a Murine Model. Biol Reprod. 2016;94:39 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 4b immunocytochemistry; mouse; fig 3d	Dey A, Robitaille M, Remke M, Maier C, Malhotra A, Gregorieff A, et al. YB-1 is elevated in medulloblastoma and drives proliferation in Sonic hedgehog-dependent cerebellar granule neuron progenitor cells and medulloblastoma cells. Oncogene. 2016;35:4256-68 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; fig 1	Zuckermann M, Hovestadt V, Knobbe Thomsen C, Zapatka M, Northcott P, Schramm K, et al. Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nat Commun. 2015;6:7391 pubmed publisher
	Matsuo A, Yoshida T, Yasukawa T, Miki R, Kume K, Kume S. Epiplakin1 is expressed in the cholangiocyte lineage cells in normal liver and adult progenitor cells in injured liver. Gene Expr Patterns. 2011;11:255-62 pubmed publisher

product information

Catalog Number :

NA03-200UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-PCNA (Ab-1) Mouse mAb (PC10)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

5111 (Hu), 18538 (Ms), 25737 (Rt)

Host Name :

Clone :

PC10

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-PCNA (Ab-1) Mouse mAb (PC10)

Cross Reactivity :

Human, Mouse, Rat, Yeast, Zebrafish

Background :

The proliferating cell nuclear antigen (PCNA) is a 37 kDa molecular weight protein also known as cyclin. The protein has also been identified as the polymerase Δ accessory protein and is detected in a cell cycle dependent manner. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cells fixed with aldehydes, the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on-going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells.

ALT Names :

MGC8367, Pcna/cyclin, PCNAR, proliferating cell nuclear antigen

Immunogen :

recombinant PCNA

Specificity :

Recognizes the ~37 kDa PCNA protein.

Package Size :

200 ug

Uses :

WB, IF, IH(P)

Storage :

Do not freeze