MSN Antibody, clone 2C12 | EMD Millipore MABT456 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MSN Antibody, clone 2C12

catalog :

MABT456

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2C12

The same clone is also sold as:

OriGene: AM06738PU-N
Novus Biologicals: NBP2-37509
ProMab: 30338
Abnova: MAB12992
Invitrogen: MA5-17130
LifeSpan Biosciences: LS-B8945

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

citations: 7

Reference
Parrondo R, de las Pozas A, Reiner T, Perez Stable C. ABT-737, a small molecule Bcl-2/Bcl-xL antagonist, increases antimitotic-mediated apoptosis in human prostate cancer cells. Peerj. 2013;1:e144 pubmed publisher
Chiang K, Yeh C, Chen S, Shen S, Hsu J, Yeh T, et al. MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1?,25-Dihydroxyvitamin D(3) in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells. Evid Based Complement Alternat Med. 2012;2012:310872 pubmed publisher
Zhou C, Pan W, Wang X, Chen T. Artesunate induces apoptosis via a Bak-mediated caspase-independent intrinsic pathway in human lung adenocarcinoma cells. J Cell Physiol. 2012;227:3778-86 pubmed publisher
Zhang W, Wang X, Chen T. Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells. Cell Signal. 2012;24:1037-46 pubmed publisher
Ji J, Tyurina Y, Tang M, Feng W, Stolz D, Clark R, et al. Mitochondrial injury after mechanical stretch of cortical neurons in vitro: biomarkers of apoptosis and selective peroxidation of anionic phospholipids. J Neurotrauma. 2012;29:776-88 pubmed publisher
Van Humbeeck C, Cornelissen T, Hofkens H, Mandemakers W, Gevaert K, De Strooper B, et al. Parkin interacts with Ambra1 to induce mitophagy. J Neurosci. 2011;31:10249-61 pubmed publisher
Rudner J, Elsaesser S, Müller A, Belka C, Jendrossek V. Differential effects of anti-apoptotic Bcl-2 family members Mcl-1, Bcl-2, and Bcl-xL on celecoxib-induced apoptosis. Biochem Pharmacol. 2010;79:10-20 pubmed publisher

product information

Catalog Number :

MABT456

Subcategory :

Cell Structure

Product Name :

Anti-MSN Antibody, clone 2C12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P26038

Host Name :

Mouse

Antigen :

MSN

Clone :

2C12

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-MSN Antibody, clone 2C12

Cross Reactivity :

Human

Background :

Moesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. The MSN encoded protein moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, the MSN encoded protein is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. The MSN encoded Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane. EMD-Millipore’s Anti-MSN, clone 2C12 has been tested in western blots against purified recombinant protein as well as multiple human cancer cell lysates. The clone has also been tested successfully in paraffin embedded immunohistochemistry on human colon tissue and by flow cytometry on human Jurkat cells and ELISA using purified recombinant antigen.

ALT Names :

Moesin;Membrane-organizing extension spike protein

Immunogen :

Purified protein fragment of human MSN expressed in E. coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry;Flow Cytometry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.