Integrin Beta1 Antibody, clone 8E3 | EMD Millipore MABT199 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin Beta1 Antibody, clone 8E3

catalog :

MABT199

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8E3

reactivity :

human

application :

western blot, immunoprecipitation

product information

Catalog Number :

MABT199

Subcategory :

Cell Structure

Product Name :

Anti-Integrin Beta1 Antibody, clone 8E3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05556

Host Name :

Mouse

Antigen :

Integrin Beta1

Clone :

8E3

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Integrin Beta1 Antibody, clone 8E3

Cross Reactivity :

Human

Background :

Integrin beta1 is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation.

ALT Names :

Integrin beta-1;Fibronectin receptor subunit beta;VLA-4 subunit beta;CD29

Immunogen :

Purified Integrin Beta1 from HT-1080 cells.

Specificity :

This antibody recognizes the N-terminus of the PSI domain of Integrin Beta1. Under native conditions, clone 8E3 is shown to recognize the unbent, monomeric ER form of Integrin Beta1 at the PSI domain; however, the epitope only becomes exposed in the Golgi form after denaturation. This observation suggests that the epitopes are present in monomeric beta 1-integrins, but become lost following assembly with alpha-integrin subunits (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.; Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.)

Package Size :

100 µL

Uses :

Western Blotting;Activity Assay;Immunoprecipitation

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.