PARG Antibody, clone D8B10 | EMD Millipore MABS61 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PARG Antibody, clone D8B10

catalog :

MABS61

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D8B10

reactivity :

human

application :

western blot, immunoprecipitation

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; fig 3 western blot; human; 1:1000; fig 3	Watanabe Y, Papoutsoglou P, Maturi V, Tsubakihara Y, Hottiger M, Heldin C, et al. Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation. J Biol Chem. 2016;291:12706-23 pubmed publisher
western blot; human; 1:2000; fig 5.b	Lovero K, Fukata Y, Granger A, Fukata M, Nicoll R. The LGI1-ADAM22 protein complex directs synapse maturation through regulation of PSD-95 function. Proc Natl Acad Sci U S A. 2015;112:E4129-37 pubmed publisher
western blot; human; 1:2000; fig 5.b	Koh D, Powell D, Blake S, Hoffman J, Hopkins M, Feng X. Enhanced cytotoxicity in triple-negative and estrogen receptorâ€‘positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncol Rep. 2015;34:1589-98 pubmed publisher
western blot; human; 1:1,000	Zhang Y, Wang J, Ding M, Yu Y. Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome. Nat Methods. 2013;10:981-4 pubmed publisher
	Molloy Simard V, St Laurent J, Vigneault F, Gaudreault M, Dargis N, Guérin M, et al. Altered expression of the poly(ADP-ribosyl)ation enzymes in uveal melanoma and regulation of PARG gene expression by the transcription factor ERM. Invest Ophthalmol Vis Sci. 2012;53:6219-31 pubmed publisher
	Grady S, Hwang J, Vastag L, Rabinowitz J, Shenk T. Herpes simplex virus 1 infection activates poly(ADP-ribose) polymerase and triggers the degradation of poly(ADP-ribose) glycohydrolase. J Virol. 2012;86:8259-68 pubmed publisher

product information

Catalog Number :

MABS61

Subcategory :

Cell Signaling

Product Name :

Anti-PARG Antibody, clone D8B10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q86W56

Host Name :

Mouse

Antigen :

PARG

Clone :

D8B10

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-PARG Antibody, clone D8B10

Cross Reactivity :

Human

Background :

Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme possessing both endo- and exoglycosidase activity against poly (ADP-ribose) (PARP), rapidly degrading PARP to release large quantities of free ADP-ribose. PARG is involved in several cellular processes including; apoptosis, DNA repair, cell cycle progression, cell survival and cellular differentiation. During apoptosis, PARG is cleaved by caspase-3 suggesting that PARG activity is regulated during this process. Although encoded by one gene, PARG is present in different cellular localizations as different isoforms: Isoform 1 (111 kDa) is present in the nucleus, Isoform 2 (102 kDA) is in the cytoplasm and Isoform 3 (99 kDa) is mitochondrial. Studies have indicated a critical role for PARG isoform 1 in the quality of sperm chromatin, and subsequent embryonic survival.

ALT Names :

poly (ADP-ribose) glycohydrolase;poly(ADP-ribose) glycohydrolase 60 kDa isoform

Immunogen :

Histidine-tagged recombinant protein corresponding to human PARG.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at 2-8°C from date of receipt