pan polyglycylated Tubulin Antibody, clone AXO 49 | EMD Millipore MABS276 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

pan polyglycylated Tubulin Antibody, clone AXO 49

catalog :

MABS276

quantity :

50 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AXO 49

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:5000; loading ...; fig 3c	Vedelek V, Laurinyecz B, Kovacs A, Juhasz G, Sinka R. Testis-Specific Bb8 Is Essential in the Development of Spermatid Mitochondria. PLoS ONE. 2016;11:e0161289 pubmed publisher
	Laurinyecz B, Vedelek V, Kovacs A, Szilasi K, Lipinszki Z, Slezák C, et al. Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm. PLoS Genet. 2019;15:e1007987 pubmed publisher
	Glenn S, Geyer P. Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1. G3 (Bethesda). 2019;9:345-357 pubmed publisher
	Duan T, Geyer P. Spermiogenesis and Male Fertility Require the Function of Suppressor of Hairy-Wing in Somatic Cyst Cells of Drosophila. Genetics. 2018;209:757-772 pubmed publisher

product information

Catalog Number :

MABS276

Subcategory :

Cell Signaling

Product Name :

Anti-pan polyglycylated Tubulin Antibody, clone AXO 49

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

pan polyglycylated Tubulin

Clone :

AXO 49

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-pan polyglycylated Tubulin Antibody, clone AXO 49

Cross Reactivity :

Drosophila;Pig;Snail;Sea Urchin;Mouse;A Broad Range Of Species

Background :

Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments indicates that polyglycylation levels of tubulin is highly regulated at the cell level. The AXO49 antibody is reactive mainly on axonemal tubulin of motile cilia and flagella which are polyglycylated. In some cases, reactivity may also be observed on other very stable microtubule networks. This antibody is useful for cilia and flagella detection.

Immunogen :

Insoluble fraction of Paramecium cilia (Paramecium axonemes)

Specificity :

This antibody recognizes the lateral connecting branches of a polymer, which contains at least 3 glycine residues. This antibody demonstrates cross reactivity against polyglycylated tubulin, as well as with other polyglycylated proteins .

Package Size :

50 µL

Uses :

Western Blotting;Immunofluorescence;Dot Blot

Storage :

Stable for 1 year at 2-8°C from date of receipt