EMD Millipore

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

antibody

Anti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, rabbit monoclonal

MABS175

100 µL

monoclonal

rabbit

nonconjugated

EP2358Y

western blot, immunocytochemistry

MABS175

Cell Signaling

Anti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, rabbit monoclonal

Antibodies

Monoclonal Antibody

Q8IVT5

Rabbit

phospho KSR1 (Ser404)

EP2358Y

Diluted Culture Supernatant

IgG

Anti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, rabbit monoclonal

Human

Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (1). MARK3 phosphorylates serine 309 and serine 404 on KSR1. While, PPP2CA dephosphorylates serine 404 on KSR1. MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signaling, indicating a link between two important developmental pathways (2).

kinase suppressor of ras 1;kinase suppressor of ras

Synthetic phospho-peptide corresponding to human KSR1 phosphorylated Ser404.

This antibody recognizes KSR1 phosphorylated at Ser404.

100 µL

Western Blotting;Immunocytochemistry

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.