Iba1/AIF1 Antibody | EMD Millipore MABN92 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Iba1/AIF1 Antibody

catalog :

MABN92

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

20A12.1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:800; loading ...; fig 6e	Ye X, Zhu M, Che X, Wang H, Liang X, Wu C, et al. Lipopolysaccharide induces neuroinflammation in microglia by activating the MTOR pathway and downregulating Vps34 to inhibit autophagosome formation. J Neuroinflammation. 2020;17:18 pubmed publisher
western blot; mouse; 1:200; loading ...; fig 5a	di Meco A, Pratico D. Early-life exposure to high-fat diet influences brain health in aging mice. Aging Cell. 2019;18:e13040 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 4d	Larabee C, Desai S, Agasing A, Georgescu C, Wren J, Axtell R, et al. Loss of Nrf2 exacerbates the visual deficits and optic neuritis elicited by experimental autoimmune encephalomyelitis. Mol Vis. 2016;22:1503-1513 pubmed
western blot; human; loading ...; fig 2b	Janer A, Prudent J, Paupe V, Fahiminiya S, Majewski J, Sgarioto N, et al. SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. EMBO Mol Med. 2016;8:1019-38 pubmed publisher
immunohistochemistry; mouse; 1:50; loading ...; fig 5d	Larabee C, Hu Y, Desai S, Georgescu C, Wren J, Axtell R, et al. Myelin-specific Th17 cells induce severe relapsing optic neuritis with irreversible loss of retinal ganglion cells in C57BL/6 mice. Mol Vis. 2016;22:332-41 pubmed
immunohistochemistry; mouse; 1:100; fig 1	Lee K, Lee H, Lin H, Tsay H, Tsai F, Shyue S, et al. Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation. 2016;13:92 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200; fig 2	Larabee C, Georgescu C, Wren J, Plafker S. Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons. BMC Neurosci. 2015;16:76 pubmed publisher
immunohistochemistry; human; fig 3	Satoh J, Kino Y, Asahina N, Takitani M, Miyoshi J, Ishida T, et al. TMEM119 marks a subset of microglia in the human brain. Neuropathology. 2016;36:39-49 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000	Gabrusiewicz K, Hossain M, Cortes Santiago N, Fan X, Kaminska B, Marini F, et al. Macrophage Ablation Reduces M2-Like Populations and Jeopardizes Tumor Growth in a MAFIA-Based Glioma Model. Neoplasia. 2015;17:374-84 pubmed publisher
immunohistochemistry; rat; 1:300 immunohistochemistry; mouse; 1:300	Li Y, Korgaonkar A, Swietek B, Wang J, Elgammal F, Elkabes S, et al. Toll-like receptor 4 enhancement of non-NMDA synaptic currents increases dentate excitability after brain injury. Neurobiol Dis. 2015;74:240-53 pubmed publisher
western blot; rat; 1:500	Barragán Iglesias P, Pineda Farias J, Cervantes Durán C, Bravo Hernández M, Rocha González H, Murbartián J, et al. Role of spinal P2Y6 and P2Y11 receptors in neuropathic pain in rats: possible involvement of glial cells. Mol Pain. 2014;10:29 pubmed publisher
immunocytochemistry; mouse; 1:200	Singh V, Mitra S, Sharma A, Gera R, Ghosh D. Isolation and characterization of microglia from adult mouse brain: selected applications for ex vivo evaluation of immunotoxicological alterations following in vivo xenobiotic exposure. Chem Res Toxicol. 2014;27:895-903 pubmed publisher
western blot; mouse; 1:250	Kaushik D, Thounaojam M, Kumawat K, Gupta M, Basu A. Interleukin-1? orchestrates underlying inflammatory responses in microglia via Krüppel-like factor 4. J Neurochem. 2013;127:233-44 pubmed publisher
immunohistochemistry; rat; 1:2000	Sogn C, Puchades M, Gundersen V. Rare contacts between synapses and microglial processes containing high levels of Iba1 and actin--a postembedding immunogold study in the healthy rat brain. Eur J Neurosci. 2013;38:2030-40 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Sheu J, Liao W, Wu U, Shyu L, Mai F, Chen L, et al. Resveratrol suppresses calcium-mediated microglial activation and rescues hippocampal neurons of adult rats following acute bacterial meningitis. Comp Immunol Microbiol Infect Dis. 2013;36:137-48 pubmed publisher
immunohistochemistry - frozen section; rat; 1:2000	Ziebell J, Taylor S, Cao T, Harrison J, Lifshitz J. Rod microglia: elongation, alignment, and coupling to form trains across the somatosensory cortex after experimental diffuse brain injury. J Neuroinflammation. 2012;9:247 pubmed publisher
	Barbeito Andrés J, Pezzuto P, Higa L, Dias A, Vasconcelos J, Santos T, et al. Congenital Zika syndrome is associated with maternal protein malnutrition. Sci Adv. 2020;6:eaaw6284 pubmed publisher
	Lauretti E, Nenov M, Dincer O, Iuliano L, Pratico D. Extra virgin olive oil improves synaptic activity, short-term plasticity, memory, and neuropathology in a tauopathy model. Aging Cell. 2020;19:e13076 pubmed publisher
	Furusho A, Aoki H, Ohno Urabe S, Nishihara M, Hirakata S, Nishida N, et al. Involvement of B Cells, Immunoglobulins, and Syk in the Pathogenesis of Abdominal Aortic Aneurysm. J Am Heart Assoc. 2018;7: pubmed publisher
	Ohno Urabe S, Aoki H, Nishihara M, Furusho A, Hirakata S, Nishida N, et al. Role of Macrophage Socs3 in the Pathogenesis of Aortic Dissection. J Am Heart Assoc. 2018;7: pubmed publisher
	Ziebell J, Ray Jones H, Lifshitz J. Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury. Neuroscience. 2017;359:209-223 pubmed publisher
	Guo Y, Johnson M, Mehrabian Z, Mishra M, Kannan R, Miller N, et al. Dendrimers Target the Ischemic Lesion in Rodent and Primate Models of Nonarteritic Anterior Ischemic Optic Neuropathy. PLoS ONE. 2016;11:e0154437 pubmed publisher
	Liu Y, Liu X, Hao W, Decker Y, Schomburg R, FÃ¼lÃ¶p L, et al. IKKÎ² deficiency in myeloid cells ameliorates Alzheimer's disease-related symptoms and pathology. J Neurosci. 2014;34:12982-99 pubmed publisher
	Hayes C, Dey D, Palavicini J, Wang H, Patkar K, Minond D, et al. Striking reduction of amyloid plaque burden in an Alzheimer's mouse model after chronic administration of carmustine. BMC Med. 2013;11:81 pubmed publisher

product information

Catalog Number :

MABN92

Subcategory :

Neuroscience

Product Name :

Anti-Iba1/AIF1 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P55008

Host Name :

Mouse

Antigen :

Iba1/AIF1

Clone :

20A12.1

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Iba1/AIF1 Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

The allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma (IFN-gamma) inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.

ALT Names :

Allograft inflammatory factor 1;AIF-1;Ionized calcium-binding adapter molecule 1;Protein G1

Immunogen :

Linear peptide corresponding to human Iba1/AIF1.

Package Size :

100 μg

Uses :

Western Blotting;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt