DCX Antibody, clone 2G5 | EMD Millipore MABN707 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

DCX Antibody, clone 2G5

catalog :

MABN707

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G5

The same clone is also sold as:

Novus Biologicals: NBP2-37476
Abcam: ab135349
LifeSpan Biosciences: LS-B8946
Invitrogen: MA5-17066
Abnova: MAB12993
ProMab: 30381

reactivity :

African green monkey, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; African green monkey; 1:200; fig 5	He J, Xiang Z, Zhu X, Ai Z, Shen J, Huang T, et al. Neuroprotective Effects of 7, 8-dihydroxyflavone on Midbrain Dopaminergic Neurons in MPP+-treated Monkeys. Sci Rep. 2016;6:34339 pubmed publisher
	Rhodes C, Zunino G, Huang S, Cardona S, Cardona A, Berger M, et al. Region specific knock-out reveals distinct roles of chromatin modifiers in adult neurogenic niches. Cell Cycle. 2018;17:377-389 pubmed publisher
	Foret M, Sandstrom R, Rhodes C, Wang Y, Berger M, Lin C. Molecular targets of chromatin repressive mark H3K9me3 in primate progenitor cells within adult neurogenic niches. Front Genet. 2014;5:252 pubmed publisher
	Sandstrom R, Foret M, Grow D, Haugen E, Rhodes C, Cardona A, et al. Epigenetic regulation by chromatin activation mark H3K4me3 in primate progenitor cells within adult neurogenic niche. Sci Rep. 2014;4:5371 pubmed publisher

product information

Catalog Number :

MABN707

Subcategory :

Neuroscience

Product Name :

Anti-DCX Antibody, clone 2G5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O43602

Host Name :

Mouse

Antigen :

DCX

Clone :

2G5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-DCX Antibody, clone 2G5

Cross Reactivity :

Human;Mouse

Background :

Doublecortin (DCX) or alternatively known as Doublin or Lissencephalin-X (Lis-X) and encoded by the human gene named DCX/DBCN/LISX is a microtubule associated protein that is required for the initial steps of neuronal dispersion and lamination during cortex brain development. Expressed by neuronal precursor cells and immature neurons, DCX expression is decreased by the time neurons are mature and express the mature neuronal marker NeuN. Thus DCX expression is a hallmark of developing neurons and neurogenesis. DCX expression is localized in the cytoplasm and along microtubule tracks. DCX appears to stabilize microtubules and causes bundling of them in the cytoplasm. In disease, DCX is the cause for an X-linked lissencephaly and subcortical laminar heterotopia, a disorder that is characterized by a “smooth” brain with few folds like normal brains and poorly formed cortical layers as well as other abnormalities. Besides developmental effects DCX may potentially help in the suppression of gliomas because in brain tumor stem cells (BTSC) self-renewal ability was significantly reduced in cells expressing DCX compared to control cells. EMD-Millipore’s Anti-DCX mouse monoclonal has been tested in western blot against recombinant protein as well as mouse heart lysate. The antibody has also been successfully tested in paraffin embedded immunohistochemistry on normal human brain and kidney cancer tissue sections as well as in fluorescent immunocytochemistry on HepG2 cells in culture and flow cytometry on SK-N-SH cells in culture and direct ELISA on varying concentrations of antigen.

ALT Names :

Neuronal migration protein doublecortin;Doublin;Lissencephalin-X;Lis-X;DCX

Immunogen :

Purified recombinant fragment of human DCX expressed in E. Coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry;Immunocytochemistry;Flow Cytometry

Storage :

Stable for 1 year at 2-8°C from date of receipt