Metadherin Antibody, clone 2F11C3 | EMD Millipore MABN699 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Metadherin Antibody, clone 2F11C3

catalog :

MABN699

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2F11C3

The same clone is also sold as:

Invitrogen: MA5-15564
Cell Signaling Technology: 9596
OriGene: AM06379PU-N
Abnova: MAB10817
ProMab: 20407
LifeSpan Biosciences: LS-B4118
Novus Biologicals: NBP1-51585

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

product information

Catalog Number :

MABN699

Subcategory :

Neuroscience

Product Name :

Anti-Metadherin Antibody, clone 2F11C3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q86UE4

Host Name :

Mouse

Antigen :

Metadherin

Clone :

2F11C3

Conjugate :

Affinity Purified

Isotype :

IgG1

Product Description :

Anti-Metadherin Antibody, clone 2F11C3

Cross Reactivity :

Human

Background :

Metadherin, or more commonly known as LYRIC, or AEG-1 (Astrocyte elevated gene-1) is a single pass membrane protein that plays a key role in anchorage-independent growth of immortalized melanocytes and astrocytes. Anchorage-independent division is a critical component in tumor cell expansion and metastasis. Similarly, metadherin activates the nuclear factor kappa- B (NF-kappa-B) transcription leading to expression of numerous genes involved in cell growth and expansion, and it is also involved in HIF-1alpha mediated angiogenesis in tumor growth. Finally new research demonstrates that metadherin interacts with RNA-induced silencing complex (RISC) and plays a very important role in RISC and miRNA functions as well. The protein is a key target of research because of its tumor promoting effects in lung, bone and brain cancers. Metadherin is localized to the endoplasmic reticulum and nuclear membranes in normal tissue, thus staining is perinuclear primarily but Metadherin staining can also be seen in the nucleus, at tight junctions and even at the cell surface in certain cancers. Metadherin expression is activated by TNF, and metadherin is expressed in many cells types with the highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Metadherin is often over expressed in various cancers including breast, brain, prostate, and melanoma and glioblastoma multiforme. EMD-Millipore’s Anti-metadherin mouse monoclonal, clone 2F11C3 has been tested by western blot against several human cancer cell lysates, by flow cytometry against human HeLa cells, and in human paraffin-embedded human liver tissue.

ALT Names :

Protein LYRIC;3D3/LYRIC;Astrocyte elevated gene-1 protein;AEG-1;Lysine-rich CEACAM1 co-isolated protein;Metadherin;Metastasis adhesion protein

Immunogen :

Purified protein fragment of Human Metadherin expressed in E. coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry;Flow Cytometry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.