CRTC2 Antibody, clone 5B10 | EMD Millipore MABN695 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CRTC2 Antibody, clone 5B10

catalog :

MABN695

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B10

The same clone is also sold as:

Invitrogen: MA5-15710
Novus Biologicals: NBP1-51665
OriGene: AM06534SU-N
ProMab: 30120
Abnova: MAB10615
LifeSpan Biosciences: LS-C169270

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog Number :

MABN695

Subcategory :

Neuroscience

Product Name :

Anti-CRTC2 Antibody, clone 5B10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q53ET0

Host Name :

Mouse

Antigen :

CRTC2

Clone :

5B10

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-CRTC2 Antibody, clone 5B10

Cross Reactivity :

Human

Background :

CREB-regulated transcription coactivator 2 (CRTC2) is a transcriptional coactivator for CREB1 which is a cAMP (CRE) site responder. CRTC2 is also known as TORC-2 (transducer of regulated cAMP response element-binding protein 2) and CRTC2 regulates gluconeogenesis as well as steroidogenic processes, and is also a coactivator for mitochondrial biogenesis in muscle cells. Localization is cytoplasmic but relocates to the nucleus in response to cAMP and glucagon levels. CRTC2 is highly expressed in thymus, spleen, muscle, and lung; it is also expressed in various cancers as well as insulinomas. Low levels of expression are found in the brain, colon, heart and kidney, with the lowest levels being in the liver and pancreas. Activity is regulated via phosphorylation. CRTC2 is inactive when phosphorylated (meaning that is does not bind CREB and assist in gene transcription) and active when dephosphorylated. EMD-Millipore’s Anti-CRTC2 mouse monoclonal, clone 5B10, has been tested in western blots against human HEK292 and HeLa cell extracts, in immunohistochemistry in paraffin embedded human ovary and lung cancer tissue, and in immunofluorescent immunocytochemistry and flow cytometry on HeLa cells in culture.

ALT Names :

TORC2;TORC-2;CRTC2;CREB-regulated transcription coactivator 2;Transducer of regulated cAMP response element-binding protein 2;Transducer of CREB protein 2

Immunogen :

Purified protein fragment of human CRTC2 expressed in E. coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry;Flow Cytometry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.