GPI Antibody, clone 1B7D7 | EMD Millipore MABN691 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GPI Antibody, clone 1B7D7

catalog :

MABN691

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1B7D7

The same clone is also sold as:

Invitrogen: MA5-15396
Abcam: ab66340
Abnova: MAB10351
Santa Cruz Biotechnology: sc-130067
LifeSpan Biosciences: LS-B4092
ProMab: 20198
Novus Biologicals: NBP1-47523
MyBioSource: MBS246581
OriGene: AM06224SU-N

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 1

Reference
Paul A, Crow M, Raudales R, He M, Gillis J, Huang Z. Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity. Cell. 2017;171:522-539.e20 pubmed publisher

product information

Catalog Number :

MABN691

Subcategory :

Neuroscience

Product Name :

Anti-GPI Antibody, clone 1B7D7

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P06744

Host Name :

Mouse

Antigen :

GPI

Clone :

1B7D7

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-GPI Antibody, clone 1B7D7

Cross Reactivity :

Human;Rat

Background :

Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore’s Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture.

ALT Names :

Glucose-6-phosphate isomerase;Autocrine motility factor;Neuroleukin;Phosphoglucose isomerase;Phosphohexose isomerase;Sperm antigen 36

Immunogen :

Purified recombinant fragment of human GPI expressed in E. Coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry;Immunofluorescence

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.