CCT2 Antibody, clone 5B5C4 | EMD Millipore MABN686 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CCT2 Antibody, clone 5B5C4

catalog :

MABN686

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B5C4

The same clone is also sold as:

ProMab: 30274
LifeSpan Biosciences: LS-C169423
Invitrogen: MA5-15909
Novus Biologicals: NBP2-37553
OriGene: AM06684SU-N
Abnova: MAB10523

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry

product information

Catalog Number :

MABN686

Subcategory :

Neuroscience

Product Name :

Anti-CCT2 Antibody, clone 5B5C4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P78371

Host Name :

Mouse

Antigen :

CCT2

Clone :

5B5C4

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-CCT2 Antibody, clone 5B5C4

Cross Reactivity :

Human;Mouse;Rat;Monkey

Background :

T-complex protein 1 subunit beta (TCP-1-beta) or alternatively known as CCT-beta which is encoded by the human gene named CCT2 or 99D8.1, is a protein that plays a role as a molecular chaperone. This means that CCT2 assists in the non-covalent folding or unfolding and the assembly or disassembly of other macromolecular structures, but does not occur in these structures when the structures are performing their normal biological functions. In the case of CCT2 it assists in the folding of various proteins including actin and tubulin, two very important cytoskeletal proteins in all cells. CCT2 is cytoplasmically expressed in mammalian cells, and CCT2 in worms at least, CCT2 is expressed in most, if not all, tissues. Moreover via inhibition assays in worms, CCT2 is required for a variety of functions including normal transgene repression and subcellular localization events, embryonic osmotic integrity, embryonic and larval viability, fertility, vulval development, and body color. In humans, CCT2 is also a component of a huge multi-protein complex called the BBS/CCT (BBSome) complex which is composed of several proteins named MKKS, BBS10, BBS12, TCP1, CCT3, CCT4, CCT5, and CCT8, that forms part of the cilia. It is thought that CCT2 may play a role in the assembly of the BBSome which is needed for ciliogenesis, and CCT2 appears to regulate the transport of vesicles to the cilia. EMD-Millipore’s Anti-CCT2 mouse monoclonal antibody has been tested in western blot against recombinant protein as well as cell lysates from HeLa, MCF-7, Jurkat, T47, K562, A431, NIH3T3, PC-12 and COS7 cells. The antibody has also been successfully tested in fluorescent immunocytochemistry on 3T3-L1 cells and in flow cytometry on NIH3T3 cells in culture and in direct ELISA on varying concentrations of recombinant antigen.

ALT Names :

T-complex protein 1 subunit beta;CCT2

Immunogen :

Purified recombinant fragment of human CCT2 expressed in E. Coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunofluorescence;Flow Cytometry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.