BHMT Antibody, clone 8C11H5 | EMD Millipore MABN679 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BHMT Antibody, clone 8C11H5

catalog :

MABN679

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8C11H5

The same clone is also sold as:

OriGene: AM06289SU-N
LifeSpan Biosciences: LS-B4100
Abnova: MAB10502
ProMab: 20285
Invitrogen: MA5-15465

reactivity :

human

application :

western blot, immunohistochemistry

product information

Catalog Number :

MABN679

Subcategory :

Neuroscience

Product Name :

Anti-BHMT Antibody, clone 8C11H5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q93088

Host Name :

Mouse

Antigen :

BHMT

Clone :

8C11H5

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-BHMT Antibody, clone 8C11H5

Cross Reactivity :

Human

Background :

Betaine, (trimethylglycine) is an organic osmolyte that is available for cells by either choline oxidase-catalyzed synthesis or concentrative uptake by the betaine/GABA transport system (BGT-1). The intracellular accumulation of betaine protects cells from different kinds of stress including long-lasting dehydration, ischemia-reperfusion, and heat shock. Betaine has been considered a “chemical chaperone” because it prevents protein denaturation under adverse conditions, a property that likely contributes to the cytoprotective effects of this osmolyte. Betaine--homocysteine S-methyltransferase 1, or betaine-homocysteine methyltransferase (BHMT) is the enzyme that is responsible for the metabolic elimination of betaine and homocysteine via their conversion to dimethylglycine and methionine. As such BHMT is one of a handful of key enzymes keenly responsible for betaine levels. BHMT is expressed at high levels in the human liver and kidney, localizing in the ER and cytoplasmic regions. BHMT expression is largely regulated at the level of the gene. BHMT activity becomes essential when folate-dependent remethylation is impaired because of nutritional deficiencies as it then provides the source for methionine. Deficiencies in BHMT can lead a variety of disorders ranging from vascular disease to neural tube birth defects such as spina bifida, fatty liver disease and cirrhosis all which can be linked to betaine metabolism disorders. EMD-Millipore’s Anti-BHMT mouse monoclonal antibody, clone 635, has been tested in western blots using recombinantly expressed BHMT cDNA and by immunohistochemistry on paraffin embedded human liver tissue samples.

ALT Names :

Betaine--homocysteine S-methyltransferase 1

Immunogen :

Purified recombinant fragment of BHMT expressed in E. coli.

Package Size :

100 µL

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.