DLK1 Antibody, clone 3A10 | EMD Millipore MABN671 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

DLK1 Antibody, clone 3A10

catalog :

MABN671

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A10

The same clone is also sold as:

OriGene: AM06690SU-N
ProMab: 30280
LifeSpan Biosciences: LS-C169429
Invitrogen: MA5-15915
Abnova: MAB10627

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry

product information

Catalog Number :

MABN671

Subcategory :

Neuroscience

Product Name :

Anti-DLK1 Antibody, clone 3A10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P80370

Host Name :

Mouse

Antigen :

DLK1

Clone :

3A10

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-DLK1 Antibody, clone 3A10

Cross Reactivity :

Human;Mouse

Background :

Protein delta homolog 1or DLK-1 (DLK1), also known as pG2 and the Preadipocyte factor-1 (Pref-1), is a single pass type 1 membrane protein known to play a steering role in neuroendocrine differentiation. The protein is expressed within the stromal cells in contact with the vascular structure of the placental villi, yok sac, fetal liver, adrenal cortex and pancreas during development and DLK1 is also expressed within the beta cells of the islets of Langerhans in the adult. DLK1 plays an important gene regulatory expression role during the development of not only these very early structures but also in the regulation of the development of many specialized cells and tissues such as adipocytes. In adipose tissue, DLK1/Pref-1 is specifically expressed in preadipocytes but not in adipocytes and thus can be used as a preadipocyte marker. The inhibition operates through the creation of a soluble cleaved cytoplasmic form of DLK1 via the action of the metalloproteinase ADAM17 on mature DLK1. Fetal Antigen 1 (FA1) or soluble DLK-1/Pref-1 activates the MAPK kinase/ERK pathway, and via Sox9 interactions ultimately inhibits the differentiation of pre-adipocytes into mature adipocytes. Furthermore, by inducing Sox9, Pref-1 promotes chondrogenic induction of mesenchymal cells but prevents chondrocyte maturation as well as osteoblast differentiation. Thus, DLK1/Pref-1 directs multipotent mesenchymal cells (MSCs) toward the chondrogenic lineage but inhibits differentiation into adipocytes as well as osteoblasts and chondrocytes. Naturally, DLK1 has multiple isoforms each with slightly differing but related influences on MSCs development. Finally, DLK1 is also expressed in various tumors including small cell lung carcinoma and numerous neuroendocrine cancers.

ALT Names :

Protein delta homolog 1;DLK-1;FA1;ZOG;pG2;PREF1;Pref-1

Immunogen :

Purified recombinant fragment of human DLK1 expressed in E. Coli.

Package Size :

100 µL

Uses :

Immunohistochemistry;Immunofluorescence

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.