Dicer1 Antibody, clone N167/7 | EMD Millipore MABN461 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Dicer1 Antibody, clone N167/7

catalog :

MABN461

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N167/7

The same clone is also sold as:

Active Motif: 39817
BioLegend: 820201
Neuromab: 73-196, 75-196
Abcam: ab167444

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, western blot knockout validation

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; loading ...; fig 3d	Eckenfelder A, Segeral E, Pinzón N, Ulveling D, Amadori C, Charpentier M, et al. Argonaute proteins regulate HIV-1 multiply spliced RNA and viral production in a Dicer independent manner. Nucleic Acids Res. 2017;45:4158-4173 pubmed publisher
	Aryal N, Pant V, Wasylishen A, Parker Thornburg J, Baseler L, El Naggar A, et al. Constitutive Dicer1 phosphorylation accelerates metabolism and aging in vivo. Proc Natl Acad Sci U S A. 2019;116:960-969 pubmed publisher
	Wang Y, Mercier R, Hobman T, LaPointe P. Regulation of RNA interference by Hsp90 is an evolutionarily conserved process. Biochim Biophys Acta. 2013;1833:2673-2681 pubmed publisher

product information

Catalog Number :

MABN461

Subcategory :

Neuroscience

Product Name :

Anti-Dicer1 Antibody, clone N167/7

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q8R418

Host Name :

Mouse

Antigen :

Dicer1

Clone :

N167/7

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Dicer1 Antibody, clone N167/7

Cross Reactivity :

Rat;Mouse;Human

Background :

RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

ALT Names :

Endoribonuclease Dicer;Double-strand-specific ribonuclease mDCR-1

Immunogen :

Recombinant protein corresponding to mouse Dicer1.

Package Size :

100 μg

Uses :

Western Blotting;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt