Aggregated a-Synuclein Antibody, clone 5G4 | EMD Millipore MABN389 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Aggregated a-Synuclein Antibody, clone 5G4

catalog :

MABN389

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5G4

reactivity :

human, rat, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; fruit fly ; loading ...; fig 7i	Ordonez D, Lee M, Feany M. α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton. Neuron. 2018;97:108-124.e6 pubmed publisher
immunohistochemistry; rat; 1:1000; loading ...; fig s6a, s6b	Roberts H, Schneider B, Brown D. α-Synuclein increases β-amyloid secretion by promoting β-/γ-secretase processing of APP. PLoS ONE. 2017;12:e0171925 pubmed publisher
	Sun L, Lian Y, Ding J, Meng Y, Li C, Chen L, et al. The role of chaperone-mediated autophagy in neurotoxicity induced by alpha-synuclein after methamphetamine exposure. Brain Behav. 2019;9:e01352 pubmed publisher
	Nakamura K, Mori F, Kon T, Tanji K, Miki Y, Tomiyama M, et al. Filamentous aggregations of phosphorylated Î±-synuclein in Schwann cells (Schwann cell cytoplasmic inclusions) in multiple system atrophy. Acta Neuropathol Commun. 2015;3:29 pubmed publisher

product information

Catalog Number :

MABN389

Subcategory :

Neuroscience

Product Name :

Anti-Aggregated a-Synuclein Antibody, clone 5G4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P37840

Host Name :

Mouse

Antigen :

SNCA

Clone :

5G4

Conjugate :

Affinity Purified

Purification Method :

Affinity purified

Isotype :

IgG1κ

Product Description :

Anti-Aggregated a-Synuclein Antibody, clone 5G4

Cross Reactivity :

Human

Background :

α-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing. Pepscan epitope mapping experiments showed that the epitope of 5G4 seems to be hidden inside full-length a-synuclein in aqueous solution, and this fact could depend on a specific conformation of the molecule. Thus, after conformational change, like b-sheet building, the binding structure of 5G4 may be accessible in biological samples. This antibody can be used as a sandwich ELISA pair with Cat. No. MABN633, Anti-a-Synuclein, clone 10D2.

ALT Names :

Aggregated a-synuclein, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP

Immunogen :

KLH-conjugated linear peptide corresponding to human Aggregated a-Synuclein.

Package Size :

100 μg

Uses :

Western Blotting, Immunohistochemistry, ELISA

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.