peptidyl-citrulline, clone F95 | EMD Millipore MABN328 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

peptidyl-citrulline, clone F95

catalog :

MABN328

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F95

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; loading ...; fig 5c western blot; human; 1:1000; loading ...; fig 5a	Casanova V, Sousa F, Shakamuri P, Svoboda P, Buch C, D Acremont M, et al. Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Front Immunol. 2020;11:85 pubmed publisher
western blot; mouse; 1:50; loading ...; fig 4a	Wizeman J, Mohan R. Expression of peptidylarginine deiminase 4 in an alkali injury model of retinal gliosis. Biochem Biophys Res Commun. 2017;487:134-139 pubmed publisher
immunohistochemistry - paraffin section; human; 1:250; fig 6	Turunen S, Huhtakangas J, Nousiainen T, Valkealahti M, Melkko J, Risteli J, et al. Rheumatoid arthritis antigens homocitrulline and citrulline are generated by local myeloperoxidase and peptidyl arginine deiminases 2, 3 and 4 in rheumatoid nodule and synovial tissue. Arthritis Res Ther. 2016;18:239 pubmed
immunohistochemistry - frozen section; mouse; 1:200; fig 4 western blot; mouse; 1:500; fig 1	Wizeman J, Nicholas A, Ishigami A, Mohan R. Citrullination of glial intermediate filaments is an early response in retinal injury. Mol Vis. 2016;22:1137-1155 pubmed
	Hollingsworth T, Radic M, Beranova Giorgianni S, Giorgianni F, Wang Y, Iannaccone A. Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4). Invest Ophthalmol Vis Sci. 2018;59:3808-3815 pubmed publisher
	Caprariello A, Rogers J, Morgan M, Hoghooghi V, Plemel J, Koebel A, et al. Biochemically altered myelin triggers autoimmune demyelination. Proc Natl Acad Sci U S A. 2018;115:5528-5533 pubmed publisher
	Walker J, Bleaken B, Romisher A, Alnwibit A, Menko A. In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype. Mol Biol Cell. 2018;29:1555-1570 pubmed publisher

product information

Catalog Number :

MABN328

Subcategory :

Neuroscience

Product Name :

Anti-peptidyl-citrulline, clone F95

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Clone :

F95

Conjugate :

Purified

Purification Method :

Purified by ion-exchange chromatography

Isotype :

IgMκ

Product Description :

Anti-peptidyl-citrulline, clone F95

Cross Reactivity :

Human, Mouse, Chicken, Rat

Background :

Deimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.

ALT Names :

Peptidyl-citrulline, Protein-bound citrulline

Immunogen :

Citrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.

Package Size :

100 μg

Uses :

Immunohistochemistry, Western Blotting, Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt