NeuN (rabbit monoclonal) Antibody, clone 27-4 | EMD Millipore MABN140 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NeuN (rabbit monoclonal) Antibody, clone 27-4

catalog :

MABN140

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

27-4

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig 4e	Qian Q, Liu Q, Zhou D, Pan H, Liu Z, He F, et al. Brain-specific ablation of Efr3a promotes adult hippocampal neurogenesis via the brain-derived neurotrophic factor pathway. FASEB J. 2017;31:2104-2113 pubmed publisher
immunocytochemistry; mouse; loading ...; fig s1	He J, Zhou R, Wu Z, Carrasco M, Kurshan P, Farley J, et al. Prevalent presence of periodic actin-spectrin-based membrane skeleton in a broad range of neuronal cell types and animal species. Proc Natl Acad Sci U S A. 2016;113:6029-34 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500; fig 2	Jara J, Stanford M, Zhu Y, Tu M, Hauswirth W, Bohn M, et al. Healthy and diseased corticospinal motor neurons are selectively transduced upon direct AAV2-2 injection into the motor cortex. Gene Ther. 2016;23:272-82 pubmed publisher
immunohistochemistry - frozen section; rat; 1:500; fig 9	Bean L, Kumar A, Rani A, Guidi M, Rosario A, Cruz P, et al. Re-Opening the Critical Window for Estrogen Therapy. J Neurosci. 2015;35:16077-93 pubmed publisher
immunohistochemistry; rat; 1:1000	Scofield M, Boger H, Smith R, Li H, Haydon P, Kalivas P. Gq-DREADD Selectively Initiates Glial Glutamate Release and Inhibits Cue-induced Cocaine Seeking. Biol Psychiatry. 2015;78:441-51 pubmed publisher
	Vetreno R, Bohnsack J, Kusumo H, Liu W, Pandey S, Crews F. Neuroimmune and epigenetic involvement in adolescent binge ethanol-induced loss of basal forebrain cholinergic neurons: Restoration with voluntary exercise. Addict Biol. 2020;25:e12731 pubmed publisher
	Long Q, Luo Q, Wang K, Bates A, Shetty A. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. Aging Dis. 2017;8:301-313 pubmed publisher
	Severson K, Xu D, Van de Loo M, Bai L, Ginty D, O Connor D. Active Touch and Self-Motion Encoding by Merkel Cell-Associated Afferents. Neuron. 2017;94:666-676.e9 pubmed publisher
	Schlamp C, Montgomery A, Mac Nair C, Schuart C, Willmer D, Nickells R. Evaluation of the percentage of ganglion cells in the ganglion cell layer of the rodent retina. Mol Vis. 2013;19:1387-96 pubmed
	Wei X, Na X, Liao G, Chen Q, Cui Y, Chen F, et al. The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection. Exp Neurol. 2013;241:159-68 pubmed publisher

product information

Catalog Number :

MABN140

Subcategory :

Neuroscience

Product Name :

Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Rabbit

Antigen :

NeuN (rabbit monoclonal)

Clone :

27-4

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

Cross Reactivity :

Mouse;Rat

Background :

NeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Immunogen :

Recombinant protein corresponding to mouse NeuN.

Package Size :

100 µL

Uses :

Immunocytochemistry;Western Blotting;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt