CD27 Antibody (human), PE-Cy7, clone O323 | EMD Millipore MABF402 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CD27 Antibody (human), PE-Cy7, clone O323

catalog :

MABF402

quantity :

100 tests

clonality :

monoclonal

host :

mouse

conjugate :

PE/Cy7

clone name :

O323

The same clone is also sold as:

LifeSpan Biosciences: LS-C40962, LS-C107092
Abnova: MAB10864
Invitrogen: 14-0279-82, 67-0279-42, 14-0279-95, 48-0279-41, 15-0279-41, 63-0279-41, 25-0279-42, 48-0279-42, 13-0279-82, 61-0279-42, 78-0279-42, 14-0279-80, 25-0279-41, 11-0279-42, 17-0279-42, 78-0279-41, 64-0279-42, 46-0279-42, 17-0279-41, 63-0279-42, 12-0279-42, 15-0279-42, 56-0279-41, 62-0279-42, 14-0279-37, 62-0279-41, 47-0279-42, 46-0279-41, 56-0279-42
OriGene: TA320253
Rockland Immunochemicals: 200-326-N65, 200-302-N65, 200-308-N65
BioLegend: 302818, 302814, 302831, 302807, 302828, 302804, 302839, 302845, 302842, 302819, 302832, 302808, 302834, 302823, 302805, 302809, 302815, 302820, 302837, 302825, 302843, 302802, 302830, 302849, 302829, 302856, 302827, 302806, 302838, 302824, 302835, 302816, 302813, 302846, 302853, 302855, 302810, 302836, 302851, 302847, 302850, 302812, 302833, 302844, 302822, 302817, 302821

reactivity :

human

application :

flow cytometry

product information

Catalog Number :

MABF402

Subcategory :

Inflammation & Immunology

Product Name :

Anti-CD27 Antibody (human), PE-Cy7, clone O323

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P26842

Host Name :

Mouse

Antigen :

CD27

Clone :

O323

Conjugate :

PE-Cy7

Purification Method :

Size Exclusion Chromatography

Isotype :

IgG1κ

Product Description :

Anti-CD27 Antibody (human), PE-Cy7, clone O323

Cross Reactivity :

Human

Background :

The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.

ALT Names :

CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD antigen CD27

Immunogen :

Immunogen information for this antibody is proprietary information.

Package Size :

100 tests

Uses :

Flow Cytometry

Storage :

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.