CD45 (human) Antibody, AlexaFluor®488, clone HI30 | EMD Millipore MABF323 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CD45 (human) Antibody, AlexaFluor®488, clone HI30

catalog :

MABF323

quantity :

100 tests

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

HI30

The same clone is also sold as:

LifeSpan Biosciences: LS-C40812
Rockland Immunochemicals: 200-302-N68, 200-326-N68, 200-308-N68
Invitrogen: 35-0459-41, MHCD4530, 12-0459-42, 69-0459-41, 13-0459-80, 69-0459-42, 48-0459-42, MHCD4505-4, 48-0459-41, 14-0459-95, 13-0459-82, MHCD4515, 17-0459-42, 12-0459-41, MHCD4517, 606-0459-42, MHCD4530TR, Q10156, Q10051, 51-0459-42, MHCD4501, 47-0459-41, 61-0459-42, 505-0459-41, MHCD4512, 58-0459-41, 505-0459-42, 11-0459-42, 11-0459-41, 14-0459-82, Q22154, MHCD4506-5, 58-0459-42, 61-0459-41, MHCD4518, 25-0459-42, 45-0459-42, 51-0459-41, MHCD4504, MHCD4501-4, 15-0459-42, 47-0459-42, MHCD4527, MHCD4522, 606-0459-41, 25-0459-41, MHCD4506, 46-0459-42, MHCD4505, MHCD4504-4, MHCD4526, 17-0459-41, MHCD4529, 46-0459-41, 45-0459-41, 35-0459-42, 14-0459-37, MHCD4528, MHCD4520
Novus Biologicals: NBP1-79127
BD Biosciences: 561864, 560976, 555480, 557748, 560367, 560973, 561866, 560566, 564357, 563204, 562279, 555481, 560368, 555482, 555485, 560779, 562312, 564914, 555483, 555484, 560915, 563879, 563792, 560975, 563791, 561865, 560974, 564105, 560777
Exbio: T9-684-T025, PB-684-T100, 11-684-C025, PO-684-T025, PO-684-T100, 11-684-C100, T9-684-T100, PB-684-T025
BioLegend: 304056, 304015, 304075, 304004, 304008, 304054, 304043, 304020, 304025, 304037, 304044, 304006, 304029, 304048, 304062, 304003, 304028, 304001, 304068, 304066, 304035, 304027, 304064, 304034, 982310, 982308, 304017, 304010, 982312, 304002, 982318, 304077, 304033, 304032, 304074, 304041, 304060, 304018, 304078, 304014, 304051, 304005, 982316, 304045, 304022, 304038, 304009, 304049, 304016, 304036, 304039, 304042, 982314, 304031, 304052, 304021, 304073, 304061, 304011, 304070, 982304, 304023, 304019, 304071, 304047, 304026, 304012, 304007, 304024, 304058, 304076, 982306, 304050
Tonbo Biosciences: 65-0459
Bio-Rad: MCA1719C, MCA1719GA, MCA1719PE, MCA1719F
Abnova: MAB13943, MAB13936, MAB13940, MAB13942, MAB13938, MAB13941, MAB13937, MAB13939, MAB12226

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

citations: 4

Reference
Mei H, Leipold M, Schulz A, Chester C, Maecker H. Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol. 2015;194:2022-31 pubmed publisher
Cuerquis J, Romieu Mourez R, Francois M, Routy J, Young Y, Zhao J, et al. Human mesenchymal stromal cells transiently increase cytokine production by activated T cells before suppressing T-cell proliferation: effect of interferon-? and tumor necrosis factor-? stimulation. Cytotherapy. 2014;16:191-202 pubmed publisher
Ishii W, Liepnieks J, Yamada T, Benson M, Kluve Beckerman B. Human SAA1-derived amyloid deposition in cell culture: a consistent model utilizing human peripheral blood mononuclear cells and serum-free medium. Amyloid. 2013;20:61-71 pubmed publisher
Campeau P, Rafei M, Boivin M, Sun Y, Grabowski G, Galipeau J. Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome. Blood. 2009;114:3181-90 pubmed publisher

product information

Catalog Number :

MABF323

Subcategory :

Inflammation & Immunology

Product Name :

Anti-CD45 (human) Antibody, AlexaFluor®488, clone HI30

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P08575

Host Name :

Mouse

Antigen :

CD45 (human)

Clone :

HI30

Conjugate :

AlexaFluor®488

Isotype :

IgG1κ

Product Description :

Anti-CD45 (human) Antibody, AlexaFluor®488, clone HI30

Cross Reactivity :

Human

Background :

CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.

ALT Names :

Receptor-type tyrosine-protein phosphatase C;Leukocyte common antigen;L-CA;T200;CD45

Immunogen :

Immunogen information on this antibody is proprietary information.

Package Size :

100 tests

Uses :

Flow Cytometry;Immunohistochemistry;Western Blotting

Storage :

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.