CD3ε (mouse) Antibody, APC, clone 145-2C11 | EMD Millipore MABF316 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CD3ε (mouse) Antibody, APC, clone 145-2C11

catalog :

MABF316

quantity :

100 μg

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

145-2C11

The same clone is also sold as:

Miltenyi Biotec: 130-092-973, 130-102-793, 130-093-179, 130-101-990, 130-102-600, 130-121-440, 130-102-794, 130-102-359, 130-102-549, 130-102-791, 130-102-496, 130-102-792, 130-101-940
LifeSpan Biosciences: LS-C21526, LS-C21522, LS-C112612, LS-C122846, LS-C357691, LS-C34550, LS-C62562, LS-C148323, LS-C21527, LS-C357689, LS-C45012, LS-C112430, LS-C148326, LS-C196284, LS-C357652, LS-C357651, LS-C195595, LS-C357688, LS-C127316, LS-C112611, LS-C62561, LS-C196283, LS-C62819, LS-C112613, LS-C105774, LS-C86802, LS-C21521, LS-C21523
OriGene: CL001P, CL001, CL001A, AM12132LE-N, CL001B, TA320270, AM12132PU-N, CL001FX, AM12132FC-N, CL001PX, AM12132AC-N, AM12132RP-N, CL001F, CL001BX
Tonbo Biosciences: 30-0031
Invitrogen: 14-0031-82, 48-0031-82, 15-0031-82, 16-0031-81, 45-0031-82, 13-0031-86, 12-0031-82, 17-0031-63, 12-0031-85, 62-0031-82, 53-0031-82, MA5-17656, 47-0031-82, 47-0031-80, 14-0031-85, 15-0031-81, 45-0031-80, 61-0031-80, 17-0031-83, 12-0031-83, MA1-10186, 11-0031-86, 13-0031-81, 16-0031-025, A14714, 11-0031-81, 17-0031-82, 25-0031-81, MA1-84019, 16-0031-85, 16-0031-050, 11-0031-85, MA5-17658, 17-0031-81, 13-0031-82, MA1-10187, MA5-17655, 25-0031-82, 14-0031-86, 11-0031-82, MA1-10184, 63-0031-82, 35-0031-82, 16-0031-86, 61-0031-82, 15-0031-83, 48-0031-80, 13-0031-85, 12-0031-81, 16-0031-82, 78-0031-82, 16-0031-38, 36-0031-85, 53-0031-80, 35-0031-80
Novus Biologicals: NBP2-52641-0.2mg, NBP2-52693-0.2mg
R&D Systems: MAB484-100
Abnova: MAB9826, MAB15455, MAB5907, MAB3220, MAB15454, MAB5909, MAB5911, MAB12205, MAB15456
Bio-Rad: MCA2690GA, MCA2690A488, MCA2690T, MCA2690
BD Biosciences: 553066, 553063, 551163, 562600, 550275, 553059, 561100, 552774, 561108, 553057, 561827, 553064, 553062, 553067, 553060, 553065, 563565, 561826, 561089, 557596, 553058, 561042, 557306, 557666, 561825, 561824, 553061, 562286, 562332
Exbio: 11-578-C100, 1P-578-C025, 1P-578-C100, PC-578-C025, 1A-578-C100, T7-578-C100, 12-578-C100, T7-578-C025, PC-578-C100, 1B-578-C100, 11-578-C025, T9-578-C025, 1A-578-C025, 1F-578-C100, T9-578-C100
Santa Cruz Biotechnology: sc-18871, sc-18871 L
Absolute Antibody: Ab00105-1.4, Ab00105-6.1, Ab00105-1.1, Ab00105-6.4, Ab00105-23.0, Ab00105-10.0, Ab00105-22.0
Rockland Immunochemicals: 200-B02-M69, 200-B08-M69
Bio X Cell: BP0001-1-5MG, BP0001-1-25MG, BE0001-1-50MG, BP0001-1-100MG, BE0001-1-1MG, BE0001-1-100MG, BP0001-1-50MG, BE0001-1-5MG, BE0001-1-25MG
BioLegend: 100351, 100348, 100353, 100327, 100367, 100371, 100349, 100328, 100362, 100325, 100330, 100307, 100347, 100329, 100339, 100364, 100303, 100368, 100304, 100326, 100322, 100310, 100360, 100372, 100341, 100306, 100312, 100301, 100320, 100308, 100321, 100334, 100359, 100363, 100365, 100345, 100361, 100340, 100324, 100311, 100302, 100355, 100305, 100336, 100366, 100309, 100335, 100333, 100319
Emfret Analytics: 70-0031
MilliporeSigma: SAB4700555, SAB4700553

reactivity :

mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

citations: 7

Reference
Textor A, Schmidt K, Kloetzel P, Weißbrich B, Perez C, Charo J, et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J Exp Med. 2016;213:2333-2348 pubmed
Shin H, Tilahun M, Cho O, Chandiran K, Kuksin C, Keerthivasan S, et al. NOTCH1 Can Initiate NF-?B Activation via Cytosolic Interactions with Components of the T Cell Signalosome. Front Immunol. 2014;5:249 pubmed publisher
Licata L, Nguyen C, Burga R, Falanga V, Espat N, Ayala A, et al. Biliary obstruction results in PD-1-dependent liver T cell dysfunction and acute inflammation mediated by Th17 cells and neutrophils. J Leukoc Biol. 2013;94:813-23 pubmed publisher
San Luis B, Sondgeroth B, Nassar N, Carpino N. Sts-2 is a phosphatase that negatively regulates zeta-associated protein (ZAP)-70 and T cell receptor signaling pathways. J Biol Chem. 2011;286:15943-54 pubmed publisher
Boding L, Nielsen M, Bonefeld C, von Essen M, Nielsen B, Lauritsen J, et al. Polymorphisms of the T cell receptor CD3delta and CD3epsilon chains affect anti-CD3 antibody binding and T cell activation. Mol Immunol. 2010;47:2450-7 pubmed publisher
Carpino N, Chen Y, Nassar N, Oh H. The Sts proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways. Mol Immunol. 2009;46:3224-31 pubmed publisher
Corre I, Gomez M, Vielkind S, Cantrell D. Analysis of thymocyte development reveals that the GTPase RhoA is a positive regulator of T cell receptor responses in vivo. J Exp Med. 2001;194:903-14 pubmed

product information

Catalog Number :

MABF316

Subcategory :

Inflammation & Immunology

Product Name :

Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P22646

Host Name :

Armenian Hamster

Antigen :

CD3ε (mouse)

Clone :

145-2C11

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

Cross Reactivity :

Mouse

Background :

CD3, also known as T3, is a member of the Ig and the ITAM superfamilies. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon and zeta. CD3 is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The CD3 antigen is present in early thymocytes, and has been considered the best all-around T cell marker. The positive staining of this marker may represent a sign of early commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies. CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

ALT Names :

T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e

Immunogen :

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

Package Size :

100 μg

Uses :

Flow Cytometry;Immunohistochemistry;Activity Assay;Immunoprecipitation;Western Blotting

Storage :

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.