EGFR Antibody, clone 225 (Azide-free) | EMD Millipore MABF120 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EGFR Antibody, clone 225 (Azide-free)

catalog :

MABF120

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

225

The same clone is also sold as:

Invitrogen: MA5-12880
LifeSpan Biosciences: LS-C88000, LS-C88001, LS-C6560
MilliporeSigma: E2156

reactivity :

human, dogs

application :

immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 5

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; fig s5c	Shaffer S, Dunagin M, Torborg S, Torre E, Emert B, Krepler C, et al. Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance. Nature. 2017;546:431-435 pubmed publisher
immunocytochemistry; human; loading ...; fig 4c immunocytochemistry; dogs; loading ...; fig 6c	Greenwood E, Maisel S, Ebertz D, Russ A, Pandey R, SCHROEDER J. Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration. Oncotarget. 2016;7:60776-60792 pubmed publisher
immunoprecipitation; human; fig 2	Bollu L, Katreddy R, Blessing A, Pham N, Zheng B, Wu X, et al. Intracellular activation of EGFR by fatty acid synthase dependent palmitoylation. Oncotarget. 2015;6:34992-5003 pubmed publisher
blocking or activating experiments; human; 2 ug/ml	Joannes A, Grelet S, Duca L, Gilles C, Kileztky C, Dalstein V, et al. Fhit regulates EMT targets through an EGFR/Src/ERK/Slug signaling axis in human bronchial cells. Mol Cancer Res. 2014;12:775-83 pubmed publisher
	Buehler J, Zeltzer S, Reitsma J, Petrucelli A, Umashankar M, Rak M, et al. Opposing Regulation of the EGF Receptor: A Molecular Switch Controlling Cytomegalovirus Latency and Replication. PLoS Pathog. 2016;12:e1005655 pubmed publisher

product information

Catalog Number :

MABF120

Subcategory :

Cell Signaling

Product Name :

Anti-EGFR Antibody, clone 225 (Azide-free)

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P00533

Host Name :

Mouse

Antigen :

EGFR

Clone :

225

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-EGFR Antibody, clone 225 (Azide-free)

Cross Reactivity :

Human

Background :

The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands including EGF, EPGN, TGFA, and EREG. Ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains which allow interaction with adaptor proteins and induction of several signaling pathways including PI3-AKT; PLCγ-PKC; Notch1; and Ras-Raf. EGFR therefore contributes to a diverse range of cellular processes inlcuding cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.

ALT Names :

Epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1

Immunogen :

Partially purified human EGFR from A431 cells

Package Size :

100 μg

Uses :

Immunoprecipitation;Neutralizing

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.