phospho RNA Pol II (Ser7), clone 3D4A12 | EMD Millipore MABE955 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho RNA Pol II (Ser7), clone 3D4A12

catalog :

MABE955

quantity :

200 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3D4A12

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

product information

Catalog Number :

MABE955

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho RNA Pol II (Ser7), clone 3D4A12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

POLR2A

Clone :

3D4A12

Conjugate :

Purified

Purification Method :

Protein G purified

Isotype :

IgG2bκ

Product Description :

Anti-phospho RNA Pol II (Ser7), clone 3D4A12

Cross Reactivity :

Human, Mouse, Canine, Monkey, Rat

Background :

As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity.

ALT Names :

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RNA PolII, RNAPIIS7p

Immunogen :

KLH-conjugated linear peptide corresponding to human phospho RNA Pol II (Ser7).

Package Size :

200 μg

Uses :

Western Blotting, ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, ELISA

Storage :

Stable for 1 year at 2-8°C from date of receipt