phospho RNA Pol II (Ser5), clone 1H4B6 | EMD Millipore MABE954 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho RNA Pol II (Ser5), clone 1H4B6

catalog :

MABE954

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

1H4B6

The same clone is also sold as:

MilliporeSigma: SAB4200638

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, chromatin immunoprecipitation, ChIP-Seq

citations: 1

Reference
Lin D, Hiron T, O Callaghan C. Intragenic transcriptional interference regulates the human immune ligand MICA. EMBO J. 2018;37: pubmed publisher

product information

Catalog Number :

MABE954

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho RNA Pol II (Ser5), clone 1H4B6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

POLR2A

Clone :

1H4B6

Conjugate :

Purified

Purification Method :

Protein G purified

Isotype :

IgG2bκ

Product Description :

Anti-phospho RNA Pol II (Ser5), clone 1H4B6

Cross Reactivity :

Human, Canine, Monkey, Mouse, Rat

Background :

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity.

ALT Names :

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

Immunogen :

KLH-conjugated linear peptide corresponding to human RNA PoI II phosphorylated at Ser5.

Package Size :

100 μg

Uses :

Western Blotting, ChIP-seq, Immunocytochemistry, Enzyme Immunoassay (ELISA), Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt