BRG1 Antibody, clone 3G4 | EMD Millipore MABE60 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BRG1 Antibody, clone 3G4

catalog :

MABE60

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3G4

The same clone is also sold as:

MilliporeSigma: SAB4200641
Active Motif: 39807

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 4b	Tagal V, Wei S, Zhang W, Brekken R, Posner B, Peyton M, et al. SMARCA4-inactivating mutations increase sensitivity to Aurora kinase A inhibitor VX-680 in non-small cell lung cancers. Nat Commun. 2017;8:14098 pubmed publisher
	Cruickshank V, Sroczynska P, Sankar A, Miyagi S, Rundsten C, Johansen J, et al. SWI/SNF Subunits SMARCA4, SMARCD2 and DPF2 Collaborate in MLL-Rearranged Leukaemia Maintenance. PLoS ONE. 2015;10:e0142806 pubmed publisher
	Rao M, Matsumoto Y, Richardson M, Panneerdoss S, Bhardwaj A, Ward J, et al. Hormone-induced and DNA demethylation-induced relief of a tissue-specific and developmentally regulated block in transcriptional elongation. J Biol Chem. 2014;289:35087-101 pubmed publisher
	Joliot V, Ait Mohamed O, Battisti V, Pontis J, Philipot O, Robin P, et al. The SWI/SNF subunit/tumor suppressor BAF47/INI1 is essential in cell cycle arrest upon skeletal muscle terminal differentiation. PLoS ONE. 2014;9:e108858 pubmed publisher

product information

Catalog Number :

MABE60

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BRG1 Antibody, clone 3G4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P51532

Host Name :

Rat

Antigen :

BRG1

Clone :

3G4

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-BRG1 Antibody, clone 3G4

Cross Reactivity :

Human;Mouse

Background :

Brahma-related gene 1 (BRG1) is a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acting as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, BRG1 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.

ALT Names :

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4;BRG1-associated factor 190A;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4;SNF2-like 4;SNF2-BETA;sucrose nonfermenting-like 4;transcription activator BRG1;brahma protein-like 1;homeotic gene regulator;global transcription activator homologous sequence;Protein brahma homolog 1;BRM/SWI2-related gene 1;Protein BRG-1;nuclear protein GRB1;Mitotic growth and transcription activator;ATP-dependent helicase SMARCA4;SNF2-beta

Immunogen :

GST-tagged recombinant protein corresponding to human BRG1.

Package Size :

100 μg

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt