pan Ago Antibody, clone 2A8 | EMD Millipore MABE56 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

pan Ago Antibody, clone 2A8

catalog :

MABE56

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A8

The same clone is also sold as:

MilliporeSigma: SAB4800048
Rockland Immunochemicals: 200-301-V04
Novus Biologicals: NBP2-59159

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, RNA immunoprecipitation

citations: 19

Published Application/Species/Sample/Dilution	Reference
RNA immunoprecipitation; mouse; loading ...; fig st4, st5	Xu H, Du X, Xu J, Zhang Y, Tian Y, Liu G, et al. Pancreatic β cell microRNA-26a alleviates type 2 diabetes by improving peripheral insulin sensitivity and preserving β cell function. PLoS Biol. 2020;18:e3000603 pubmed publisher
RNA immunoprecipitation; mouse; fig s6	Parsi S, Smith P, Goupil C, Dorval V, HÃ©bert S. Preclinical Evaluation of miR-15/107 Family Members as Multifactorial Drug Targets for Alzheimer's Disease. Mol Ther Nucleic Acids. 2015;4:e256 pubmed publisher
immunoprecipitation; mouse; fig 2	Alexander M, Hu R, Runtsch M, Kagele D, Mosbruger T, Tolmachova T, et al. Exosome-delivered microRNAs modulate the inflammatory response to endotoxin. Nat Commun. 2015;6:7321 pubmed publisher
immunoprecipitation; human; fig 4	Guo Y, Liu J, Elfenbein S, Ma Y, Zhong M, Qiu C, et al. Characterization of the mammalian miRNA turnover landscape. Nucleic Acids Res. 2015;43:2326-41 pubmed publisher
chromatin immunoprecipitation; human	Pohlers M, Calabrese J, Magnuson T. Small RNA expression from the human macrosatellite DXZ4. G3 (Bethesda). 2014;4:1981-9 pubmed publisher
immunoprecipitation; human	Maute R, Schneider C, Sumazin P, Holmes A, Califano A, Basso K, et al. tRNA-derived microRNA modulates proliferation and the DNA damage response and is down-regulated in B cell lymphoma. Proc Natl Acad Sci U S A. 2013;110:1404-9 pubmed publisher
immunoprecipitation; mouse	Saikia M, Krokowski D, Guan B, Ivanov P, Parisien M, Hu G, et al. Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress. J Biol Chem. 2012;287:42708-25 pubmed publisher
	Saha S, Kiran M, Kuscu C, Chatrath A, Wotton D, Mayo M, et al. Long Noncoding RNA DRAIC Inhibits Prostate Cancer Progression by Interacting with IKK to Inhibit NF-κB Activation. Cancer Res. 2020;80:950-963 pubmed publisher
	Sheu Gruttadauria J, Pawlica P, Klum S, Wang S, Yario T, Schirle Oakdale N, et al. Structural Basis for Target-Directed MicroRNA Degradation. Mol Cell. 2019;75:1243-1255.e7 pubmed publisher
	Orellana E, AbdElaal A, Rangasamy L, Tenneti S, Myoung S, Low P, et al. Enhancing MicroRNA Activity through Increased Endosomal Release Mediated by Nigericin. Mol Ther Nucleic Acids. 2019;16:505-518 pubmed publisher
	Frank A, Ebersberger S, Fink A, Lampe S, Weigert A, Schmid T, et al. Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype. Nat Commun. 2019;10:1135 pubmed publisher
	Guan T, Dominguez C, Amezquita R, Laidlaw B, Cheng J, Henao Mejia J, et al. ZEB1, ZEB2, and the miR-200 family form a counterregulatory network to regulate CD8+ T cell fates. J Exp Med. 2018;215:1153-1168 pubmed publisher
	Hao P, Waxman D. Functional Roles of Sex-Biased, Growth Hormone-Regulated MicroRNAs miR-1948 and miR-802 in Young Adult Mouse Liver. Endocrinology. 2018;159:1377-1392 pubmed publisher
	Bu Y, Yoshida A, Chitnis N, Altman B, Tameire F, Oran A, et al. A PERK-miR-211 axis suppresses circadian regulators and protein synthesis to promote cancer cell survival. Nat Cell Biol. 2018;20:104-115 pubmed publisher
	Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. 2016;17:94 pubmed publisher
	Bellutti F, Kauer M, Kneidinger D, Lion T, Klein R. Identification of RISC-associated adenoviral microRNAs, a subset of their direct targets, and global changes in the targetome upon lytic adenovirus 5 infection. J Virol. 2015;89:1608-27 pubmed publisher
	Jacobs M, Jeffers L, Welch A, Wingo C, Cain B. MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells. Life Sci. 2014;118:195-9 pubmed publisher
	Jiang C, Croft A, Tseng H, Guo S, Jin L, Hersey P, et al. Repression of microRNA-768-3p by MEK/ERK signalling contributes to enhanced mRNA translation in human melanoma. Oncogene. 2014;33:2577-88 pubmed publisher
	Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J. Efficient and specific gene knockdown by small interfering RNAs produced in bacteria. Nat Biotechnol. 2013;31:350-6 pubmed publisher

product information

Catalog Number :

MABE56

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-pan Ago Antibody, clone 2A8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UKV8

Host Name :

Mouse

Antigen :

pan Ago

Clone :

2A8

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-pan Ago Antibody, clone 2A8

Cross Reactivity :

Human

Background :

Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.

ALT Names :

eukaryotic translation initiation factor 2C, 2;protein argonaute-2;argonaute 2;Protein slicer;argonaute2;PAZ Piwi domain protein;Eukaryotic translation initiation factor 2C;Argonaute2;Argonaute4;Ago4;Protein argonaute-4

Immunogen :

Histidine - tagged recombinant protein corresponding to human Ago.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt