acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 | EMD Millipore MABE351 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

catalog :

MABE351

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13HH3-1A5

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 1

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; human; loading ...; fig 7a	Li N, Li Y, Lv J, Zheng X, Wen H, Shen H, et al. ZMYND8 Reads the Dual Histone Mark H3K4me1-H3K14ac to Antagonize the Expression of Metastasis-Linked Genes. Mol Cell. 2016;63:470-84 pubmed publisher

product information

Catalog Number :

MABE351

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

acetyl-Histone H3 (Lys14)

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

Cross Reactivity :

Human;Mouse

Background :

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, acetylation of H3K4 is regulated by Gcn5 and the Mst2 complex, and is crucial to the activation of DNA damage checkpoint.

ALT Names :

Histone H3.1t;H3/t;H3t;H3/g

Immunogen :

Ovalbumin-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys14.

Package Size :

100 µL

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;ChIP-seq;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.