phospho-RNAPII (Tyr1) Antibody, clone 3D12 | EMD Millipore MABE350 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-RNAPII (Tyr1) Antibody, clone 3D12

catalog :

MABE350

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3D12

The same clone is also sold as:

Active Motif: 61383

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 2c	Mayfield J, Irani S, Escobar E, Zhang Z, Burkholder N, Robinson M, et al. Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb. elife. 2019;8: pubmed publisher
western blot; human; loading ...; fig s2f	Hancock M, Meyer R, Mistry M, Khetani R, Wagschal A, Shin T, et al. Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression. Cell. 2019;177:722-736.e22 pubmed publisher

product information

Catalog Number :

MABE350

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

phospho-RNAPII (Tyr1)

Clone :

3D12

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

Cross Reactivity :

Human

Background :

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

ALT Names :

DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1

Immunogen :

Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt