Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate | EMD Millipore MABE343-AF488 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate

catalog :

MABE343-AF488

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

12D10

reactivity :

Xenopus laevis

application :

immunocytochemistry

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; Xenopus laevis; 1:200; loading ...; fig 4b	Koppers M, Cagnetta R, Shigeoka T, Wunderlich L, Vallejo Ramirez P, Qiaojin Lin J, et al. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. elife. 2019;8: pubmed publisher
	López Erauskin J, Tadokoro T, Baughn M, Myers B, McAlonis Downes M, Chillon Marinas C, et al. ALS/FTD-Linked Mutation in FUS Suppresses Intra-axonal Protein Synthesis and Drives Disease Without Nuclear Loss-of-Function of FUS. Neuron. 2018;100:816-830.e7 pubmed publisher
	Wong H, Lin J, Ströhl F, Roque C, Cioni J, Cagnetta R, et al. RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo. Neuron. 2017;95:852-868.e8 pubmed publisher
	Konopacki F, Wong H, Dwivedy A, Bellon A, Blower M, Holt C. ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis. Open Biol. 2016;6:150218 pubmed publisher

product information

Catalog Number :

MABE343-AF488

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Clone :

12D10

Conjugate :

AlexaFluor®488

Purification Method :

Protein G purified

Product Description :

Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate

Cross Reactivity :

Human

Background :

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. This product is a conjugated version of Cat. No. MABE343.

Immunogen :

Puromycin-incorporated neosynthesized proteins at multiple molecular weights

Package Size :

100 µL

Uses :

Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt