MDM2 Antibody, clone IF2 | EMD Millipore MABE340 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDM2 Antibody, clone IF2

catalog :

MABE340

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IF2

The same clone is also sold as:

Invitrogen: 33-7100, MA1-113

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:50; fig s2 western blot; human; 3:1000; fig s7	Swetzig W, Wang J, Das G. Estrogen receptor alpha (ERÎ±/ESR1) mediates the p53-independent overexpression of MDM4/MDMX and MDM2 in human breast cancer. Oncotarget. 2016;7:16049-69 pubmed publisher
western blot; human	Strauss G, Westhoff M, Fischer Posovszky P, Fulda S, Schanbacher M, Eckhoff S, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008;15:332-43 pubmed
	Zaika A, Slade N, Erster S, Sansome C, Joseph T, Pearl M, et al. DeltaNp73, a dominant-negative inhibitor of wild-type p53 and TAp73, is up-regulated in human tumors. J Exp Med. 2002;196:765-80 pubmed
	Castedo M, Ferri K, Blanco J, Roumier T, Larochette N, Barretina J, et al. Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation. J Exp Med. 2001;194:1097-110 pubmed

product information

Catalog Number :

MABE340

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-MDM2 Antibody, clone IF2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q00987

Host Name :

Mouse

Antigen :

MDM2

Clone :

IF2

Conjugate :

Purified

Isotype :

IgG2bκ

Product Description :

Anti-MDM2 Antibody, clone IF2

Cross Reactivity :

Human

Background :

MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.

ALT Names :

E3 ubiquitin-protein ligase Mdm2;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2

Immunogen :

Recombinant protein corresponding to human MDM2.

Package Size :

100 μg

Uses :

Western Blotting;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt