p21WAF1 Antibody, clone EA10 | EMD Millipore MABE325 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p21WAF1 Antibody, clone EA10

catalog :

MABE325

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EA10

The same clone is also sold as:

Invitrogen: 33-7000

reactivity :

human

application :

western blot, flow cytometry

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Martínez Torres A, Quiney C, Attout T, Boullet H, Herbi L, Vela L, et al. CD47 agonist peptides induce programmed cell death in refractory chronic lymphocytic leukemia B cells via PLCÎ³1 activation: evidence from mice and humans. PLoS Med. 2015;12:e1001796 pubmed publisher
western blot; human	Morita A, Ariyasu S, Wang B, Asanuma T, Onoda T, Sawa A, et al. AS-2, a novel inhibitor of p53-dependent apoptosis, prevents apoptotic mitochondrial dysfunction in a transcription-independent manner and protects mice from a lethal dose of ionizing radiation. Biochem Biophys Res Commun. 2014;450:1498-504 pubmed publisher
	Drummond C, Esfandiari A, Liu J, Lu X, Hutton C, Jackson J, et al. TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation. Oncotarget. 2016;7:46203-46218 pubmed publisher
	Leitch C, Osdal T, Andresen V, Molland M, Kristiansen S, Nguyen X, et al. Hydroxyurea synergizes with valproic acid in wild-type p53 acute myeloid leukaemia. Oncotarget. 2016;7:8105-18 pubmed publisher
	van den Boom V, Rozenveld Geugien M, Bonardi F, Malanga D, van Gosliga D, Heijink A, et al. Nonredundant and locus-specific gene repression functions of PRC1 paralog family members in human hematopoietic stem/progenitor cells. Blood. 2013;121:2452-61 pubmed publisher
	Huart A, MacLaine N, Narayan V, Hupp T. Exploiting the MDM2-CK1? protein-protein interface to develop novel biologics that induce UBL-kinase-modification and inhibit cell growth. PLoS ONE. 2012;7:e43391 pubmed publisher
	Ito A, Morita A, Ohya S, Yamamoto S, Enomoto A, Ikekita M. Cycloheximide suppresses radiation-induced apoptosis in MOLT-4 cells with Arg72 variant of p53 through translational inhibition of p53 accumulation. J Radiat Res. 2011;52:342-50 pubmed
	Phillips A, Teunisse A, Lam S, Lodder K, Darley M, Emaduddin M, et al. HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity. J Biol Chem. 2010;285:29111-27 pubmed publisher
	Zhuang J, Hawkins S, Glenn M, Lin K, Johnson G, Carter A, et al. Akt is activated in chronic lymphocytic leukemia cells and delivers a pro-survival signal: the therapeutic potential of Akt inhibition. Haematologica. 2010;95:110-8 pubmed publisher
	Castedo M, Ferri K, Blanco J, Roumier T, Larochette N, Barretina J, et al. Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation. J Exp Med. 2001;194:1097-110 pubmed

product information

Catalog Number :

MABE325

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p21WAF1 Antibody, clone EA10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P38936

Host Name :

Mouse

Antigen :

p21WAF1

Clone :

EA10

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-p21WAF1 Antibody, clone EA10

Cross Reactivity :

Human

Background :

The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth.

ALT Names :

Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21

Immunogen :

Recombinant protein corresponding to human p21WAF1.

Package Size :

100 μg

Uses :

Western Blotting;Flow Cytometry

Storage :

Stable for 1 year at 2-8°C from date of receipt