PCNA Antibody, clone PC10 | EMD Millipore MABE288 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PCNA Antibody, clone PC10

catalog :

MABE288

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC10

The same clone is also sold as:

Cell Signaling Technology: 8580, 2586
Abnova: MAB12946
BioLegend: 307901, 307902, 307908, 307904, 307914, 307909, 307912
MyBioSource: MBS245134
Exbio: 11-182-C100, 1P-182-T025, 1P-182-T100
Novus Biologicals: NBP2-80905, NB500-106
Absolute Antibody: Ab00605-23.0, Ab00605-2.0
Abcam: ab29
Bio-Rad: MCA1558, MCA1558F, MCA1558P
Santa Cruz Biotechnology: sc-56, sc-56 HRP, sc-56 AC
OriGene: AM10035PU-S, AM10035FC-N, TA353373L, AM10035PU-N, SM1421P, TA309795
Invitrogen: 13-3900, PCNA01, 13-3940, MA5-11355, MA5-11358
BD Biosciences: 555567, 555566
LifeSpan Biosciences: LS-C87502, LS-B3434, LS-C86889, LS-B9987, LS-C9535, LS-C170844, LS-C358014, LS-C95343, LS-C180563, LS-C87459, LS-C180561, LS-C180560, LS-C87503, LS-C86890, LS-B1887, LS-C43736
Dako: M0879
Enzo Life Sciences: ALX-801-091-L001
Acris Antibodies: SM1421P
GeneTex: GTX20029

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 29

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6a	Wilson R, Biasutto A, Wang L, Fischer R, Baple E, Crosby A, et al. PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions. DNA Repair (Amst). 2017;50:22-35 pubmed publisher
immunohistochemistry - paraffin section; zebrafish ; 1:100; fig 5	He S, Mansour M, Zimmerman M, Ki D, Layden H, Akahane K, et al. Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain. elife. 2016;5: pubmed publisher
western blot; mouse; 1:1000	Chen C, Sloane J, Li H, Aytan N, Giannaris E, Zeldich E, et al. The antiaging protein Klotho enhances oligodendrocyte maturation and myelination of the CNS. J Neurosci. 2013;33:1927-39 pubmed publisher
	Hoffmann S, Smedegaard S, Nakamura K, Mortuza G, RÃ¤schle M, IbaÃ±ez de Opakua A, et al. TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress. J Cell Biol. 2016;212:63-75 pubmed publisher
	Kuroda Y, Hayashi S, Hagio S, Abe M, Furukawa S, Nakae D. Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat. J Toxicol Pathol. 2014;27:147-51 pubmed publisher
	Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed publisher
	Gill R, Michael A, Westley L, Kocher H, Murphy J, Dhoot G. SULF1/SULF2 splice variants differentially regulate pancreatic tumour growth progression. Exp Cell Res. 2014;324:157-71 pubmed publisher
	Chen X, Paudyal S, Chin R, You Z. PCNA promotes processive DNA end resection by Exo1. Nucleic Acids Res. 2013;41:9325-38 pubmed publisher
	Edelmann K, Glashauser L, Sprungala S, Hesl B, Fritschle M, Ninkovic J, et al. Increased radial glia quiescence, decreased reactivation upon injury and unaltered neuroblast behavior underlie decreased neurogenesis in the aging zebrafish telencephalon. J Comp Neurol. 2013;521:3099-115 pubmed publisher
	Karger C, Kurtz F, Steppan D, Schwarzensteiner I, Machura K, Angel P, et al. Procollagen I-expressing renin cell precursors. Am J Physiol Renal Physiol. 2013;305:F355-61 pubmed publisher
	Niwa N, Akimoto Kato A, Sakuma M, Kuraku S, Hayashi S. Homeogenetic inductive mechanism of segmentation in polychaete tail regeneration. Dev Biol. 2013;381:460-70 pubmed publisher
	Akane H, Shiraki A, Imatanaka N, Akahori Y, Itahashi M, Ohishi T, et al. Glycidol induces axonopathy by adult-stage exposure and aberration of hippocampal neurogenesis affecting late-stage differentiation by developmental exposure in rats. Toxicol Sci. 2013;134:140-54 pubmed publisher
	Solarczyk K, Zarebski M, Dobrucki J. Inducing local DNA damage by visible light to study chromatin repair. DNA Repair (Amst). 2012;11:996-1002 pubmed publisher
	Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed publisher
	Beck H, Nähse Kumpf V, Larsen M, O Hanlon K, Patzke S, Holmberg C, et al. Cyclin-dependent kinase suppression by WEE1 kinase protects the genome through control of replication initiation and nucleotide consumption. Mol Cell Biol. 2012;32:4226-36 pubmed
	Lee K, Rhee K. Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis. Cell Cycle. 2012;11:2476-85 pubmed publisher
	Tichy E, Pillai R, Deng L, Tischfield J, Hexley P, Babcock G, et al. The abundance of Rad51 protein in mouse embryonic stem cells is regulated at multiple levels. Stem Cell Res. 2012;9:124-34 pubmed publisher
	Islam M, Fujinaga R, Yanai A, Jahan M, Takeshita Y, Kokubu K, et al. Characterization of the "sporadically lurking HAP1-immunoreactive (SLH) cells" in the hippocampus, with special reference to the expression of steroid receptors, GABA, and progenitor cell markers. Neuroscience. 2012;210:67-81 pubmed publisher
	Postnikov Y, Kurahashi T, Zhou M, Bustin M. The nucleosome binding protein HMGN1 interacts with PCNA and facilitates its binding to chromatin. Mol Cell Biol. 2012;32:1844-54 pubmed publisher
	Muscolini M, Montagni E, Palermo V, Di Agostino S, Gu W, Abdelmoula Souissi S, et al. The cancer-associated K351N mutation affects the ubiquitination and the translocation to mitochondria of p53 protein. J Biol Chem. 2011;286:39693-702 pubmed publisher
	Yang G, Hinson M, Bordner J, Lin Q, Fernando A, La P, et al. Silencing hyperoxia-induced C/EBP? in neonatal mice improves lung architecture via enhanced proliferation of alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2011;301:L187-96 pubmed publisher
	Klemm D, Majka S, Crossno J, Psilas J, Reusch J, Garat C. Reduction of reactive oxygen species prevents hypoxia-induced CREB depletion in pulmonary artery smooth muscle cells. J Cardiovasc Pharmacol. 2011;58:181-91 pubmed publisher
	Pritchard M, Malinak R, Nagy L. Early growth response (EGR)-1 is required for timely cell-cycle entry and progression in hepatocytes after acute carbon tetrachloride exposure in mice. Am J Physiol Gastrointest Liver Physiol. 2011;300:G1124-31 pubmed publisher
	Maurer C, Schonthaler H, Mueller K, Neuhauss S. Distinct retinal deficits in a zebrafish pyruvate dehydrogenase-deficient mutant. J Neurosci. 2010;30:11962-72 pubmed publisher
	Beck H, Nähse V, Larsen M, Groth P, Clancy T, Lees M, et al. Regulators of cyclin-dependent kinases are crucial for maintaining genome integrity in S phase. J Cell Biol. 2010;188:629-38 pubmed publisher
	Munro S, Khaire N, Inche A, Carr S, La Thangue N. Lysine methylation regulates the pRb tumour suppressor protein. Oncogene. 2010;29:2357-67 pubmed publisher
	Reimer M, Sörensen I, Kuscha V, Frank R, Liu C, Becker C, et al. Motor neuron regeneration in adult zebrafish. J Neurosci. 2008;28:8510-6 pubmed publisher
	Brun J, Chiu R, Lockhart K, Xiao W, Wouters B, Gray D. hMMS2 serves a redundant role in human PCNA polyubiquitination. BMC Mol Biol. 2008;9:24 pubmed publisher
	von Lukowicz T, Hassa P, Lohmann C, Boren J, Braunersreuther V, Mach F, et al. PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc Res. 2008;78:158-66 pubmed

product information

Catalog Number :

MABE288

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-PCNA Antibody, clone PC10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12004

Host Name :

Mouse

Antigen :

PCNA

Clone :

PC10

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-PCNA Antibody, clone PC10

Cross Reactivity :

Human

Background :

Proliferating cell nuclear antigen (PCNA) is a heterotrimeric nuclear protein that functions as a clamp on replicating DNA. In cases of DNA damage and failure of the DNA repair pathways, PCNA mediates translesion DNA synthesis, a DNA damage tolerance (DDT) pathway, which allows replication to progress in spite of large lesions to DNA which may be caused by replication stress, ionizing radiation, spontaneous DNA damage, among other factors. PCNA primarily recruits low-fidelity DNA polymerase enzymes that are capable of elongating DNA over lesion sites. PCNA is regulated by cycles of ubiquitination, depending on the DDT pathway involved: PCNA is monoubiquitinated by Rad6–Rad18; polyubiquitinated by the Mms2-Ubc13-Rad5; or sumoylated by the Ubc9-Siz1 complex.

ALT Names :

Proliferating cell nuclear antigen;PCNA;Cyclin

Immunogen :

Recombinant protein corresponding to human PCNA.

Package Size :

200 μg

Uses :

Western Blotting;Immunohistochemistry;Immunofluorescence

Storage :

Stable for 1 year at 2-8°C from date of receipt