Replication Protein A Antibody, clone RPA34-19 | EMD Millipore MABE286 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Replication Protein A Antibody, clone RPA34-19

catalog :

MABE286

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RPA34-19

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2a	Lai Y, Zhu M, Wu W, Rokutanda N, Togashi Y, Liang W, et al. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Sci Rep. 2019;9:14257 pubmed publisher
western blot; mouse; 5 ug/ml	Li P, Ma X, Adams I, Yuan P. A tight control of Rif1 by Oct4 and Smad3 is critical for mouse embryonic stem cell stability. Cell Death Dis. 2015;6:e1588 pubmed publisher
	Arlt M, Glover T. Inhibition of topoisomerase I prevents chromosome breakage at common fragile sites. DNA Repair (Amst). 2010;9:678-89 pubmed publisher

product information

Catalog Number :

MABE286

Subcategory :

Cell Signaling

Product Name :

Anti-Replication Protein A Antibody, clone RPA34-19

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P15927

Host Name :

Mouse

Antigen :

Replication Protein A

Clone :

RPA34-19

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Replication Protein A Antibody, clone RPA34-19

Cross Reactivity :

Human

Background :

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

ALT Names :

Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34

Immunogen :

Replication Protein A purified from U293 cells.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt