Replication Protein A Antibody, clone RPA34-20 | EMD Millipore MABE285 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Replication Protein A Antibody, clone RPA34-20

catalog :

MABE285

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RPA34-20

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 1 western blot; human; fig 3	Hoffmann S, Smedegaard S, Nakamura K, Mortuza G, RÃ¤schle M, IbaÃ±ez de Opakua A, et al. TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress. J Cell Biol. 2016;212:63-75 pubmed publisher
immunocytochemistry; human; fig 5	Van Sluis M, McStay B. A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage. Genes Dev. 2015;29:1151-63 pubmed publisher
western blot; human	Lin B, Gupta D, Heinen C. Human pluripotent stem cells have a novel mismatch repair-dependent damage response. J Biol Chem. 2014;289:24314-24 pubmed publisher
immunohistochemistry - paraffin section; mouse	Link J, Jahn D, Schmitt J, Göb E, Baar J, Ortega S, et al. The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse. PLoS Genet. 2013;9:e1003261 pubmed publisher
	Bai G, Kermi C, Stoy H, Schiltz C, Bacal J, Zaino A, et al. HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis. Mol Cell. 2020;78:1237-1251.e7 pubmed publisher
	Ghezraoui H, Oliveira C, Becker J, Bilham K, Moralli D, Anzilotti C, et al. 53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ. Nature. 2018;560:122-127 pubmed publisher
	Tanaka N, Patel A, Tang L, Silver N, Lindemann A, Takahashi H, et al. Replication Stress Leading to Apoptosis within the S-phase Contributes to Synergism between Vorinostat and AZD1775 in HNSCC Harboring High-Risk TP53 Mutation. Clin Cancer Res. 2017;23:6541-6554 pubmed publisher
	Fukumoto Y, Miura T, Morii M, Kubota S, Honda T, Kubota S, et al. v-Src inhibits the interaction between Rad17 and Rad9 and induces replication fork collapse. Biochem Biophys Res Commun. 2014;450:885-90 pubmed publisher
	Munoz M, Laulier C, Gunn A, Cheng A, Robbiani D, Nussenzweig A, et al. RING finger nuclear factor RNF168 is important for defects in homologous recombination caused by loss of the breast cancer susceptibility factor BRCA1. J Biol Chem. 2012;287:40618-28 pubmed publisher
	Forment J, Walker R, Jackson S. A high-throughput, flow cytometry-based method to quantify DNA-end resection in mammalian cells. Cytometry A. 2012;81:922-8 pubmed publisher
	Dellaire G, Ching R, Ahmed K, Jalali F, Tse K, Bristow R, et al. Promyelocytic leukemia nuclear bodies behave as DNA damage sensors whose response to DNA double-strand breaks is regulated by NBS1 and the kinases ATM, Chk2, and ATR. J Cell Biol. 2006;175:55-66 pubmed

product information

Catalog Number :

MABE285

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Replication Protein A Antibody, clone RPA34-20

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P15927

Host Name :

Mouse

Antigen :

Replication Protein A

Clone :

RPA34-20

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Replication Protein A Antibody, clone RPA34-20

Cross Reactivity :

Human

Background :

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

ALT Names :

Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34

Immunogen :

Replication Protein A purified from U293 cells

Package Size :

100 μg

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt