p53 Antibody, clone PAb421 | EMD Millipore MABE283 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p53 Antibody, clone PAb421

catalog :

MABE283

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAb421

The same clone is also sold as:

Absolute Antibody: Ab00142-2.0, Ab00142-23.0
Enzo Life Sciences: BML-SA293-0050
Novus Biologicals: NBP2-62555

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig e1b, e1d, e1e	Nassour J, Radford R, Correia A, Fusté J, Schoell B, Jauch A, et al. Autophagic cell death restricts chromosomal instability during replicative crisis. Nature. 2019;565:659-663 pubmed publisher
western blot; mouse; loading ...; fig 1a	Guo Y, Li H, Ke X, Deng M, Wu Z, Cai Y, et al. Degradation of Caytaxin Causes Learning and Memory Deficits via Activation of DAPK1 in Aging. Mol Neurobiol. 2019;56:3368-3379 pubmed publisher
western blot; mouse; 1:1000; fig 1	Safdar A, Khrapko K, Flynn J, Saleem A, De Lisio M, Johnston A, et al. Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice. Skelet Muscle. 2016;6:7 pubmed publisher
western blot; rat; 1:1000	Jullien N, Roche C, Brue T, Figarella Branger D, Graillon T, Barlier A, et al. Dose-dependent dual role of PIT-1 (POU1F1) in somatolactotroph cell proliferation and apoptosis. PLoS ONE. 2015;10:e0120010 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Yan H, Solozobova V, Zhang P, Armant O, Kuehl B, Brenner Weiss G, et al. p53 is active in murine stem cells and alters the transcriptome in a manner that is reminiscent of mutant p53. Cell Death Dis. 2015;6:e1662 pubmed publisher
western blot; mouse; 1:2000; fig s7	Suzuki D, Sahu R, Leu N, Senoo M. The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells. Development. 2015;142:282-90 pubmed publisher
immunocytochemistry; mouse; 1:500	Takada Y, Isono K, Shinga J, Turner J, Kitamura H, Ohara O, et al. Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. Development. 2007;134:579-90 pubmed
	Contadini C, Monteonofrio L, Virdia I, Prodosmo A, Valente D, Chessa L, et al. p53 mitotic centrosome localization preserves centrosome integrity and works as sensor for the mitotic surveillance pathway. Cell Death Dis. 2019;10:850 pubmed publisher
	Vennin C, Mélénec P, Rouet R, Nobis M, Cazet A, Murphy K, et al. CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan. Nat Commun. 2019;10:3637 pubmed publisher
	Ou Y, Wang S, Jiang L, Zheng B, Gu W. p53 Protein-mediated regulation of phosphoglycerate dehydrogenase (PHGDH) is crucial for the apoptotic response upon serine starvation. J Biol Chem. 2015;290:457-66 pubmed publisher
	Miki T, Matsumoto T, Zhao Z, Lee C. p53 regulates Period2 expression and the circadian clock. Nat Commun. 2013;4:2444 pubmed publisher
	Farkas C, Martins C, Escobar D, Hepp M, Castro A, Evan G, et al. Wild type p53 transcriptionally represses the SALL2 transcription factor under genotoxic stress. PLoS ONE. 2013;8:e73817 pubmed publisher
	Wang S, Yu G, Jiang L, Li T, Lin Q, Tang Y, et al. p53-Dependent regulation of metabolic function through transcriptional activation of pantothenate kinase-1 gene. Cell Cycle. 2013;12:753-61 pubmed publisher
	Takahashi R, Giannini C, Sarkaria J, Schroeder M, Rogers J, Mastroeni D, et al. p53 isoform profiling in glioblastoma and injured brain. Oncogene. 2013;32:3165-74 pubmed publisher
	Zaika A, Slade N, Erster S, Sansome C, Joseph T, Pearl M, et al. DeltaNp73, a dominant-negative inhibitor of wild-type p53 and TAp73, is up-regulated in human tumors. J Exp Med. 2002;196:765-80 pubmed
	Fortin A, Cregan S, MacLaurin J, Kushwaha N, Hickman E, Thompson C, et al. APAF1 is a key transcriptional target for p53 in the regulation of neuronal cell death. J Cell Biol. 2001;155:207-16 pubmed

product information

Catalog Number :

MABE283

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p53 Antibody, clone PAb421

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P02340

Host Name :

Mouse

Antigen :

p53

Clone :

PAb421

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-p53 Antibody, clone PAb421

Cross Reactivity :

Mouse

Background :

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kDa phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy. Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features: Most of them are missense point mutations giving rise to an altered protein function. Also, many, but not all, mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

ALT Names :

Cellular tumor antigen p53;Tumor suppressor p53

Immunogen :

Partially purified mouse p53

Specificity :

This antibody recognizes amino acids 376-378 of human p53.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.