MDM2 Antibody, clone 2A10 | EMD Millipore MABE281 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDM2 Antibody, clone 2A10

catalog :

MABE281

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A10

The same clone is also sold as:

Invitrogen: MA1-25896
Abcam: ab16895

reactivity :

human, rat

application :

western blot, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1a	Du C, Wu H, Leng R. UBE4B targets phosphorylated p53 at serines 15 and 392 for degradation. Oncotarget. 2016;7:2823-36 pubmed publisher
immunoprecipitation; human western blot; human	Leslie P, Ke H, Zhang Y. The MDM2 RING domain and central acidic domain play distinct roles in MDM2 protein homodimerization and MDM2-MDMX protein heterodimerization. J Biol Chem. 2015;290:12941-50 pubmed publisher
western blot; rat	Longman M, Ranieri A, Avkiran M, Snabaitis A. Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory class G-protein coupled receptors in cardiomyocytes. PLoS ONE. 2014;9:e86234 pubmed publisher
	Oh L, Hainaut P, Blanchet S, Ariffin H. Expression of p53 N-terminal isoforms in B-cell precursor acute lymphoblastic leukemia and its correlation with clinicopathological profiles. BMC Cancer. 2020;20:110 pubmed publisher
	Wu H, Leng R. MDM2 mediates p73 ubiquitination: a new molecular mechanism for suppression of p73 function. Oncotarget. 2015;6:21479-92 pubmed
	De Nicola F, Catena V, Rinaldo C, Bruno T, Iezzi S, Sorino C, et al. HIPK2 sustains apoptotic response by phosphorylating Che-1/AATF and promoting its degradation. Cell Death Dis. 2014;5:e1414 pubmed publisher
	Preuss K, Fadle N, Regitz E, Held G, Pfreundschuh M. Inactivation of protein-phosphatase 2A causing hyperphosphorylation of autoantigenic paraprotein targets in MGUS/MM is due to an exchange of its regulatory subunits. Int J Cancer. 2014;135:2046-53 pubmed publisher
	Lessard F, Stefanovsky V, Tremblay M, Moss T. The cellular abundance of the essential transcription termination factor TTF-I regulates ribosome biogenesis and is determined by MDM2 ubiquitinylation. Nucleic Acids Res. 2012;40:5357-67 pubmed publisher
	Buss M, Read T, Schniederjan M, Gandhi K, Castellino R. HDM2 promotes WIP1-mediated medulloblastoma growth. Neuro Oncol. 2012;14:440-58 pubmed publisher

product information

Catalog Number :

MABE281

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-MDM2 Antibody, clone 2A10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q00987

Host Name :

Mouse

Antigen :

MDM2

Clone :

2A10

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-MDM2 Antibody, clone 2A10

Cross Reactivity :

Human

Background :

MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.

ALT Names :

E3 ubiquitin-protein ligase Mdm2;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2

Immunogen :

Human MDM2 protein

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at 2-8°C from date of receipt