dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 | EMD Millipore MABE241 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12

catalog :

MABE241

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

51TA-2H1

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

product information

Catalog Number :

MABE241

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68431

Host Name :

Mouse

Antigen :

dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28)

Clone :

51TA-2H1

Conjugate :

Ascites

Isotype :

IgG1κ

Product Description :

Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12

Cross Reactivity :

Human

Background :

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Conversely,deacetylation of histone H3 lysine 9 is associated with gene-activating events. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

ALT Names :

Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/I;Histone H3/j;Histone H3/k;Histone H3/l

Immunogen :

Ovalbumin-conjugated linear peptide corresponding to human Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28.

Specificity :

This antibody recognizes Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28.

Package Size :

100 µL

Uses :

Western Blotting;Dot Blot;Immunocytochemistry;Immunoprecipitation;Immunohistochemistry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.