phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2 | EMD Millipore MABE229 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

catalog :

MABE229

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CP3.2

The same clone is also sold as:

OriGene: TA353086L

reactivity :

human

application :

western blot, immunocytochemistry

product information

Catalog Number :

MABE229

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P06493

Host Name :

Mouse

Antigen :

phospho-Cdk1 (Thr14

Clone :

CP3.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

Cross Reactivity :

Human

Background :

Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.

ALT Names :

Cyclin-dependent kinase 1;CDK1;Cell division control protein 2 homolog;Cell division protein kinase 1;p34 protein kinase

Immunogen :

Linear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Specificity :

This antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt