dimethyl Histone H3 (Lys27) Antibody, clone 8H2 | EMD Millipore MABE19 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl Histone H3 (Lys27) Antibody, clone 8H2

catalog :

MABE19

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

8H2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

MABE19

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl Histone H3 (Lys27) Antibody, clone 8H2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68431

Host Name :

Rat

Antigen :

dimethyl Histone H3 (Lys27)

Clone :

8H2

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-dimethyl Histone H3 (Lys27) Antibody, clone 8H2

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Histone modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications. These modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 can be mono-, di-, or trimethylated at Lys27 by the action of histone methyltransferases such as EZH2 or NSD3. Methylation of lysine 27 in histone H3 is commonly associated with heterochromatin. and transcriptional repression.

ALT Names :

H3K27me2;Histone H3 (di methyl K27);H3 histone family, member M;H3 histone, family 2;histone 2, H3c;histone cluster 2, H3c

Immunogen :

Linear peptide corresponding to human Histone H3 methylated at Lys27.

Specificity :

This antibody recognizes Histone H3 methylated at Lys27.

Package Size :

100 μg

Uses :

Western Blotting;ELISA;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt