5-hydroxymethylcytosine (5hmC) Antibody, clone AB3/63.3 | EMD Millipore MABE176 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

5-hydroxymethylcytosine (5hmC) Antibody, clone AB3/63.3

catalog :

MABE176

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

AB3/63.3

application :

immunocytochemistry

citations: 3

Reference
Scola G, Kim H, Young L, Salvador M, Andreazza A. Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons. Psychopharmacology (Berl). 2014;231:4189-98 pubmed publisher
Zhang Q, Lei X, Lu H. Alterations of epigenetic signatures in hepatocyte nuclear factor 4? deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays. PLoS ONE. 2014;9:e84925 pubmed publisher
Cao Z, Zhou N, Zhang Y, Zhang Y, Wu R, Li Y, et al. Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. Theriogenology. 2014;81:496-508 pubmed publisher

product information

Catalog Number :

MABE176

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone AB3/63.3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Rat

Antigen :

5-hydroxymethylcytosine (5hmC)

Clone :

AB3/63.3

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone AB3/63.3

Cross Reactivity :

All

Background :

5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

ALT Names :

5hmeC;5' hydroxymethyl cytosine;5'OH methyl cytosine;5'OHmeC;5hmc;5-hmC

Immunogen :

5-Hydroxymethylcytosine conjugated to BSA

Specificity :

This antibody detects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine.

Package Size :

100 μg

Uses :

Dot Blot;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt