Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal | EMD Millipore MABE15 product information

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal

catalog :

MABE15

quantity :

100 µL

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

EP957Y

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

EMD Millipore product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:500	Frantzi M, Zoidakis J, Papadopoulos T, Zurbig P, Katafigiotis I, Stravodimos K, et al. IMAC fractionation in combination with LC-MS reveals H2B and NIF-1 peptides as potential bladder cancer biomarkers. J Proteome Res. 2013;12:3969-79 pubmed publisher

product information

Catalog Number :

MABE15

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16778

Host Name :

Rabbit

Antigen :

Histone H2B

Clone :

EP957Y

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

ALT Names :

histone cluster 2, H2be;histone 2, H2be;H2B histone family, member Q;Histone H2B.q;Histone H2B-GL105;histone H2B type 2-E

Immunogen :

Synthetic peptide corresponding to human Histone H2B.

Specificity :

This antibody recognizes Histone H2B.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry;Immunoprecipitation

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

more info or order :

EMD Millipore product webpage