5-methylcytosine Antibody, clone 33D3 | EMD Millipore MABE146 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

5-methylcytosine Antibody, clone 33D3

catalog :

MABE146

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33D3

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, chromatin immunoprecipitation, immunohistochemistry - paraffin section, other

citations: 14

Reference
Chen D, Chao D, Rocha L, Kolar M, Nguyen Huu V, Krawczyk M, et al. The lipid elongation enzyme ELOVL2 is a molecular regulator of aging in the retina. Aging Cell. 2020;19:e13100 pubmed publisher
Crochemore C, Fernandez Molina C, Montagne B, Salles A, Ricchetti M. CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence. Nat Commun. 2019;10:5576 pubmed publisher
Fang S, Li J, Xiao Y, Lee M, Guo L, Han W, et al. Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development. Nat Commun. 2019;10:4297 pubmed publisher
Gu T, Lin X, Cullen S, Luo M, Jeong M, Estecio M, et al. DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells. Genome Biol. 2018;19:88 pubmed publisher
Lee Y, Park U, Kim E, Hwang J, Jeong J, Um S. Tumor antigen PRAME is up-regulated by MZF1 in cooperation with DNA hypomethylation in melanoma cells. Cancer Lett. 2017;403:144-151 pubmed publisher
Liu Y, Zhang B, Meng X, Korn M, Parent J, Lu L, et al. UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures. Epigenetics. 2017;12:551-560 pubmed publisher
Zhang Y, Sun X, Zhang L, Zhang W. Testicular Dnmt3 expression and global DNA methylation are down-regulated by gonadotropin releasing hormones in the ricefield eel Monopterus albus. Sci Rep. 2017;7:43158 pubmed publisher
Song Y, Liu L, Feng Y, Wei Y, Yue X, He W, et al. Chilling- and Freezing-Induced Alterations in Cytosine Methylation and Its Association with the Cold Tolerance of an Alpine Subnival Plant, Chorispora bungeana. PLoS ONE. 2015;10:e0135485 pubmed publisher
Liu D, Xiong H, Ellis A, Northrup N, Dobbin K, Shin D, et al. Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level. PLoS Genet. 2015;11:e1005277 pubmed publisher
PÃ©rez M, Viejo M, Lacuesta M, Toorop P, CaÃ±al M. Epigenetic and hormonal profile during maturation of Quercus Suber L. somatic embryos. J Plant Physiol. 2015;173:51-61 pubmed publisher
Cuna A, Halloran B, Faye Petersen O, Kelly D, Crossman D, Cui X, et al. Alterations in gene expression and DNA methylation during murine and human lung alveolar septation. Am J Respir Cell Mol Biol. 2015;53:60-73 pubmed publisher
Congras A, Yerle Bouissou M, Pinton A, Vignoles F, Liaubet L, Ferchaud S, et al. Sperm DNA methylation analysis in swine reveals conserved and species-specific methylation patterns and highlights an altered methylation at the GNAS locus in infertile boars. Biol Reprod. 2014;91:137 pubmed publisher
Scola G, Kim H, Young L, Salvador M, Andreazza A. Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons. Psychopharmacology (Berl). 2014;231:4189-98 pubmed publisher
Zhang Q, Lei X, Lu H. Alterations of epigenetic signatures in hepatocyte nuclear factor 4? deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays. PLoS ONE. 2014;9:e84925 pubmed publisher

product information

Catalog Number :

MABE146

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-5-methylcytosine Antibody, clone 33D3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

5-methylcytosine

Clone :

33D3

Conjugate :

Purified

Isotype :

IgG1λ

Product Description :

Anti-5-methylcytosine Antibody, clone 33D3

Cross Reactivity :

Human;Mouse

Background :

5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.

ALT Names :

methylcytosine;methylcytidine

Immunogen :

Ovalbumin-conjugated 5-methylcytosine

Specificity :

This antibody detects 5-Methylcytosine, but not unmethylated cytosine.

Package Size :

100 μg

Uses :

Dot Blot;Immunofluorescence;Southern Blotting;Immunohistochemistry (Paraffin);Methylated DNA Immunoprecipitation (MeDIP);Flow Cytometry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.