phospho Histone H3 (Ser28) Antibody, clone E191, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho Histone H3 (Ser28) Antibody, clone E191, rabbit monoclonal

catalog :

MABE13

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

E191

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:200; fig s4	Cases O, Joseph A, Obry A, Santin M, Ben Yacoub S, PÃ¢ques M, et al. Foxg1-Cre Mediated Lrp2 Inactivation in the Developing Mouse Neural Retina, Ciliary and Retinal Pigment Epithelia Models Congenital High Myopia. PLoS ONE. 2015;10:e0129518 pubmed publisher
	Frascaroli G, Lecher C, Varani S, Setz C, van der Merwe J, Brune W, et al. Human Macrophages Escape Inhibition of Major Histocompatibility Complex-Dependent Antigen Presentation by Cytomegalovirus and Drive Proliferation and Activation of Memory CD4+ and CD8+ T Cells. Front Immunol. 2018;9:1129 pubmed publisher
	Martin C, Ahmad I, Klingseisen A, Hussain M, Bicknell L, Leitch A, et al. Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Nat Genet. 2014;46:1283-1292 pubmed publisher

product information

Catalog Number :

MABE13

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho Histone H3 (Ser28) Antibody, clone E191, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P84243

Host Name :

Rabbit

Antigen :

phospho Histone H3 (Ser28)

Clone :

E191

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-phospho Histone H3 (Ser28) Antibody, clone E191, rabbit monoclonal

Cross Reactivity :

Human;Mouse

Background :

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4).

ALT Names :

H3 histone, family 3A;histone H3.3

Immunogen :

Phospho-synthetic peptide corresponding to human Histone H3 at Ser28.

Specificity :

This antibody only detects Histone H3 phosphorylated on Ser28.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.