HSPA5 Antibody, clone 4E3 | EMD Millipore MABC675 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

HSPA5 Antibody, clone 4E3

catalog :

MABC675

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E3

The same clone is also sold as:

Abnova: MAB10689
Invitrogen: MA5-15619
LifeSpan Biosciences: LS-B4157
ProMab: 30028

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1c	Rashid M, Coombs K. Serum-reduced media impacts on cell viability and protein expression in human lung epithelial cells. J Cell Physiol. 2019;234:7718-7724 pubmed publisher

product information

Catalog Number :

MABC675

Subcategory :

Cell Structure

Product Name :

Anti-HSPA5 Antibody, clone 4E3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P11021

Host Name :

Mouse

Antigen :

HSPA5

Clone :

4E3

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-HSPA5 Antibody, clone 4E3

Cross Reactivity :

Human;Rat;Mouse

Background :

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER. EMD-Millipore’s Anti-HSPA5 protein mouse monoclonal antibody, clone 4E3, has been tested by western blot in human HeLa and Jurkat cell lysates as well as mouse NIH3T3 cell lysates. The clone has also been successfully tested in immunohistochemistry in human, paraffin-embedded, thyroid tissues.

ALT Names :

78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP

Immunogen :

Purified recominant fragment of human HSPA5 expressed in E. coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.