GPC3 Antibody, clone 9C2 | EMD Millipore MABC667 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GPC3 Antibody, clone 9C2

catalog :

MABC667

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9C2

The same clone is also sold as:

Abcam: ab129381
Invitrogen: MA5-17083
ProMab: 30372
Novus Biologicals: NBP2-37483
OriGene: AM06776PU-N
LifeSpan Biosciences: LS-C169086

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4c	Han S, Ma X, Zhao Y, Zhao H, Batista A, Zhou S, et al. Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer. Oncotarget. 2016;7:44406-44416 pubmed publisher

product information

Catalog Number :

MABC667

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-GPC3 Antibody, clone 9C2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P51654

Host Name :

Mouse

Antigen :

GPC3

Clone :

9C2

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-GPC3 Antibody, clone 9C2

Cross Reactivity :

Human;Mouse

Background :

Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.

ALT Names :

SGB;DGSX;MXR7;SDYS;SGBS;OCI-5;SGBS1;GTR2-2;Glypican-3;Intestinal protein OCI-5

Immunogen :

Purified recombinant fragment of human GPC3 expressed in E. Coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunofluorescence;Immunohistochemistry;Flow Cytometry

Storage :

Stable for 1 year at 2-8°C from date of receipt