EIF4B Antibody, clone 1F5 | EMD Millipore MABC666 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EIF4B Antibody, clone 1F5

catalog :

MABC666

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F5

The same clone is also sold as:

Invitrogen: MA5-17071
LifeSpan Biosciences: LS-C169079
Novus Biologicals: NBP2-37488
Cell Signaling Technology: 13088
OriGene: AM06769PU-N
ProMab: 30365

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog Number :

MABC666

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-EIF4B Antibody, clone 1F5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P23588

Host Name :

Mouse

Antigen :

EIF4B

Clone :

1F5

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-EIF4B Antibody, clone 1F5

Cross Reactivity :

Human;Mouse

Background :

The eukaryotic translation initiation factor 4B (eIF4B, eIF-4b) is one of several proteins involved in the initiation of eukaryotic translation. Together with other proteins EIF4B forms a complex with the 40S ribosomal subunit and Met-tRNA that ultimately allows for translation to begin. EIF4B contains two RNA binding domains, one binds RNA and the other specifically binds the 18S portion of the small (40S) ribosomal subunit RNA. EIF4B act as an anchor and then interacts with eIF4A, a helicase to help unwind and position the RNA for translation. EIF4B must be phosphorylated on Ser422 in order for translation to occur; the phosphorylation is mediated by S6K1/S6K2 kinases and or p90S6 kinase (RSK) in a growth factor dependent manner. Activation of S6K or RSK proteins requires PDK1 phosphorylation as well, thus two great signaling pathways, the mTOR/Pi3K and MAPK pathways converge on EIF4B to control its phosphorylation and subsequent activity. EMD-Millipore’s Anti-EIF4B mouse monoclonal antibody has been tested in western blot against recombinant human EIF4B protein as well lysates from a variety of human cell lines. In addition, the antibody has been successfully tested in flow cytometry on HeLa over expressing EIF4B and in fluorescent immunocytochemistry on mouse NIH3T3 cells in culture.

ALT Names :

Eukaryotic translation initiation factor 4B;eIF-4B

Immunogen :

Purified recombinant fragment of human EIF4B expressed in E. Coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Flow Cytometry;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt