MUM1 Antibody, clone 4G10 | EMD Millipore MABC640 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MUM1 Antibody, clone 4G10

catalog :

MABC640

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G10

The same clone is also sold as:

Abnova: MAB10819
LifeSpan Biosciences: LS-B4162
ProMab: 30048
OriGene: AM06446SU-N, AM20464SU-N
Invitrogen: MA5-15639

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 32

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1e	Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2c	Manocha G, Floden A, Rausch K, Kulas J, McGregor B, Rojanathammanee L, et al. APP Regulates Microglial Phenotype in a Mouse Model of Alzheimer's Disease. J Neurosci. 2016;36:8471-86 pubmed publisher
western blot; human; loading ...; fig 7b	Carmona G, Perera U, Gillett C, Naba A, Law A, Sharma V, et al. Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE. Oncogene. 2016;35:5155-69 pubmed publisher
western blot; mouse	Cejudo Martin P, Yuen A, Vlahovich N, Lock P, Courtneidge S, Diaz B. Genetic disruption of the sh3pxd2a gene reveals an essential role in mouse development and the existence of a novel isoform of tks5. PLoS ONE. 2014;9:e107674 pubmed publisher
immunocytochemistry; human; 1:1000 western blot; human; 1:1000	Lee J, Hong W, Majeti R, Stearns T. Centrosome-kinase fusions promote oncogenic signaling and disrupt centrosome function in myeloproliferative neoplasms. PLoS ONE. 2014;9:e92641 pubmed publisher
western blot; human	Beauchamp E, Woods B, Dulak A, Tan L, Xu C, Gray N, et al. Acquired resistance to dasatinib in lung cancer cell lines conferred by DDR2 gatekeeper mutation and NF1 loss. Mol Cancer Ther. 2014;13:475-82 pubmed publisher
western blot; human	Luis B, Carpino N. Insights into the suppressor of T-cell receptor (TCR) signaling-1 (Sts-1)-mediated regulation of TCR signaling through the use of novel substrate-trapping Sts-1 phosphatase variants. FEBS J. 2014;281:696-707 pubmed publisher
immunocytochemistry; human western blot; human	Costa P, Scales T, Ivaska J, Parsons M. Integrin-specific control of focal adhesion kinase and RhoA regulates membrane protrusion and invasion. PLoS ONE. 2013;8:e74659 pubmed publisher
immunocytochemistry; human western blot; human	Kubota S, Fukumoto Y, Aoyama K, Ishibashi K, Yuki R, Morinaga T, et al. Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1? (HP1?) with heterochromatin. J Biol Chem. 2013;288:17871-83 pubmed publisher
western blot; human	Li Y, Shively J. CEACAM1 regulates Fas-mediated apoptosis in Jurkat T-cells via its interaction with ?-catenin. Exp Cell Res. 2013;319:1061-72 pubmed publisher
western blot; human	Luechapanichkul R, Chen X, Taha H, Vyas S, Guan X, Freitas M, et al. Specificity profiling of dual specificity phosphatase vaccinia VH1-related (VHR) reveals two distinct substrate binding modes. J Biol Chem. 2013;288:6498-510 pubmed publisher
western blot; human; 1:500	Janson C, Kasahara N, Prendergast G, Colicelli J. RIN3 is a negative regulator of mast cell responses to SCF. PLoS ONE. 2012;7:e49615 pubmed publisher
western blot; mouse western blot; human	Ma P, Cierniewska A, Signarvic R, Cieslak M, Kong H, Sinnamon A, et al. A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling. Blood. 2012;119:1935-45 pubmed publisher
	Protack C, Foster T, Hashimoto T, Yamamoto K, Lee M, Kraehling J, et al. Eph-B4 regulates adaptive venous remodeling to improve arteriovenous fistula patency. Sci Rep. 2017;7:15386 pubmed publisher
	Kim J, Choi K, Hong C, Park H, Choi E, Kim Y, et al. Tyr42 phosphorylation of RhoA GTPase promotes tumorigenesis through nuclear factor (NF)-?B. Free Radic Biol Med. 2017;112:69-83 pubmed publisher
	Vaishnavi A, Schubert L, Rix U, Marek L, Le A, Keysar S, et al. EGFR Mediates Responses to Small-Molecule Drugs Targeting Oncogenic Fusion Kinases. Cancer Res. 2017;77:3551-3563 pubmed publisher
	Burrell T, Divekar S, Weeber E, Rebeck G. Fyn tyrosine kinase increases Apolipoprotein E Receptor 2 levels and phosphorylation. PLoS ONE. 2014;9:e110845 pubmed publisher
	Greuber E, Pendergast A. Abl family kinases regulate Fc?R-mediated phagocytosis in murine macrophages. J Immunol. 2012;189:5382-92 pubmed publisher
	Lim Y, Lim S, Tomar A, Gardel M, Bernard Trifilo J, Chen X, et al. PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility. J Cell Biol. 2008;180:187-203 pubmed publisher
	Shvartsman D, Donaldson J, Diaz B, Gutman O, Martin G, Henis Y. Src kinase activity and SH2 domain regulate the dynamics of Src association with lipid and protein targets. J Cell Biol. 2007;178:675-86 pubmed
	Liu Y, Zhu M, Nishida K, Hirano T, Zhang W. An essential role for RasGRP1 in mast cell function and IgE-mediated allergic response. J Exp Med. 2007;204:93-103 pubmed
	Sanjuan M, Rao N, Lai K, Gu Y, Sun S, Fuchs A, et al. CpG-induced tyrosine phosphorylation occurs via a TLR9-independent mechanism and is required for cytokine secretion. J Cell Biol. 2006;172:1057-68 pubmed
	Cluzel C, Saltel F, Lussi J, Paulhe F, Imhof B, Wehrle Haller B. The mechanisms and dynamics of (alpha)v(beta)3 integrin clustering in living cells. J Cell Biol. 2005;171:383-92 pubmed
	Zhu M, Liu Y, Koonpaew S, Granillo O, Zhang W. Positive and negative regulation of FcepsilonRI-mediated signaling by the adaptor protein LAB/NTAL. J Exp Med. 2004;200:991-1000 pubmed
	Kasahara K, Nakayama Y, Ikeda K, Fukushima Y, Matsuda D, Horimoto S, et al. Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain. J Cell Biol. 2004;165:641-52 pubmed
	Woodring P, Meisenhelder J, Johnson S, Zhou G, Field J, Shah K, et al. c-Abl phosphorylates Dok1 to promote filopodia during cell spreading. J Cell Biol. 2004;165:493-503 pubmed
	Marsh H, Dubreuil C, Quevedo C, Lee A, Majdan M, Walsh G, et al. SHP-1 negatively regulates neuronal survival by functioning as a TrkA phosphatase. J Cell Biol. 2003;163:999-1010 pubmed
	Gu H, Botelho R, Yu M, Grinstein S, Neel B. Critical role for scaffolding adapter Gab2 in Fc gamma R-mediated phagocytosis. J Cell Biol. 2003;161:1151-61 pubmed
	Soriano S, Hernanz Falcón P, Rodríguez Frade J, De Ana A, Garzón R, Carvalho Pinto C, et al. Functional inactivation of CXC chemokine receptor 4-mediated responses through SOCS3 up-regulation. J Exp Med. 2002;196:311-21 pubmed
	Vang T, Torgersen K, Sundvold V, Saxena M, Levy F, Skalhegg B, et al. Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor. J Exp Med. 2001;193:497-507 pubmed
	Brdicka T, Pavlistová D, Leo A, Bruyns E, Korinek V, Angelisova P, et al. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000;191:1591-604 pubmed
	Sosinowski T, Pandey A, Dixit V, Weiss A. Src-like adaptor protein (SLAP) is a negative regulator of T cell receptor signaling. J Exp Med. 2000;191:463-74 pubmed

product information

Catalog Number :

MABC640

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-MUM1 Antibody, clone 4G10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q2TAK8

Host Name :

Mouse

Antigen :

MUM1

Clone :

4G10

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-MUM1 Antibody, clone 4G10

Cross Reactivity :

Human

Background :

Mutated melanoma-associated antigen 1 (MUM1), also known as Protein expandere and PWWP domain-containing protein MUM1. MUM1’s normal role is as a DNA and chromatin associated protein that is involved in the cell’s response to DNA damage. It is driven to DNA breaks by its interaction with another DNA chromatin protein, TP53BP1, which it binds. The binding facilitates chromatin relaxation and entry of DNA repair enzymes. MUM1 is required for efficient DNA repair and cellular survival after DNA damage. Interestingly MUM1 was first identified by cloning the chromosomal breakpoints of a multiple myeloma cell line and was identified as a member of the interferon response transcriptional factor gene family (IFR). MUM1 appears to play a critical role in cell proliferation, differentiation and survival of various cancers, as well as monocytes and dendritic cells. MUM1 is expressed in B cells and plasma cells as well and in some populations of T lymphocytes. EMD-Millipore’s Anti-MUM1 mouse monoclonal antibody has been tested in against recombinantly expressed human MUM1 protein and in paraffin embedded immunohistochemistry on human melanoma, non-Hodgkin’s lymphoma and normal uterus tissue sections.

ALT Names :

PWWP domain-containing protein MUM1;Mutated melanoma-associated antigen 1;MUM-1;Protein expandere

Immunogen :

Purified recombinant fragment of human MUM1 expressed in E. Coli.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.