MLKL Antibody, clone 3H1 | EMD Millipore MABC604 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MLKL Antibody, clone 3H1

catalog :

MABC604

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3H1

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, western blot knockout validation

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 8	Kang S, Fernandes Alnemri T, Rogers C, Mayes L, Wang Y, Dillon C, et al. Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3. Nat Commun. 2015;6:7515 pubmed publisher
western blot; mouse; 1:2000; loading ...; fig 1a	Wu Y, Chou T, Young L, Hsieh F, Pan H, Mo S, et al. Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis. 2020;11:305 pubmed publisher
western blot; mouse; loading ...; fig 4c	Krishna Subramanian S, Singer S, Armaka M, Banales J, Hölzer K, Schirmacher P, et al. RIPK1 and death receptor signaling drive biliary damage and early liver tumorigenesis in mice with chronic hepatobiliary injury. Cell Death Differ. 2019;: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1p, 5e, 6d	Benmerzoug S, Rose S, Bounab B, Gosset D, Duneau L, Chenuet P, et al. STING-dependent sensing of self-DNA drives silica-induced lung inflammation. Nat Commun. 2018;9:5226 pubmed publisher
western blot; mouse; loading ...	Peltzer N, Darding M, Montinaro A, Dráber P, Draberova H, Kupka S, et al. LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature. 2018;557:112-117 pubmed publisher
western blot; mouse; fig 4	Lin J, Kumari S, Kim C, Van T, Wachsmuth L, Polykratis A, et al. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Nature. 2016;540:124-128 pubmed publisher
immunoprecipitation; mouse; 1:50; loading ...; fig 6g	Günther C, He G, Kremer A, Murphy J, Petrie E, Amann K, et al. The pseudokinase MLKL mediates programmed hepatocellular necrosis independently of RIPK3 during hepatitis. J Clin Invest. 2016;126:4346-4360 pubmed publisher
western blot; human; loading ...; fig 5a	Heulot M, Chevalier N, Puyal J, Margue C, Michel S, Kreis S, et al. The TAT-RasGAP317-326 anti-cancer peptide can kill in a caspase-, apoptosis-, and necroptosis-independent manner. Oncotarget. 2016;7:64342-64359 pubmed publisher
western blot; mouse; loading ...; fig 4h	Shutinoski B, Alturki N, Rijal D, Bertin J, Gough P, Schlossmacher M, et al. K45A mutation of RIPK1 results in poor necroptosis and cytokine signaling in macrophages, which impacts inflammatory responses in vivo. Cell Death Differ. 2016;23:1628-37 pubmed publisher
western blot; mouse; fig 5	Tortola L, Nitsch R, Bertrand M, Kogler M, Redouane Y, Kozieradzki I, et al. The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate. Cell Rep. 2016;15:1481-1492 pubmed publisher
western blot; mouse; fig 4	Aaes T, Kaczmarek A, Delvaeye T, De Craene B, De Koker S, Heyndrickx L, et al. Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep. 2016;15:274-87 pubmed publisher
immunoprecipitation; human; fig 1 western blot; human; fig 1	Zhao X, Chen Z, Zhao J, Zhang P, Pu Y, Jiang S, et al. Hsp90 modulates the stability of MLKL and is required for TNF-induced necroptosis. Cell Death Dis. 2016;7:e2089 pubmed publisher
western blot; human; loading ...; fig 6a western blot; mouse; loading ...; fig 3a	Moriwaki K, Chan F. Regulation of RIPK3- and RHIM-dependent Necroptosis by the Proteasome. J Biol Chem. 2016;291:5948-59 pubmed publisher
western blot; mouse; fig 3 western blot; human; fig 5	Jacobsen A, Lowes K, Tanzer M, Lucet I, Hildebrand J, Petrie E, et al. HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death. Cell Death Dis. 2016;7:e2051 pubmed publisher
western blot; mouse; 1:1000; fig 6	Ogura Y, Hindi S, Sato S, Xiong G, Akira S, Kumar A. TAK1 modulates satellite stem cell homeostasis and skeletal muscle repair. Nat Commun. 2015;6:10123 pubmed publisher
	Schwarzer R, Jiao H, Wachsmuth L, Tresch A, Pasparakis M. FADD and Caspase-8 Regulate Gut Homeostasis and Inflammation by Controlling MLKL- and GSDMD-Mediated Death of Intestinal Epithelial Cells. Immunity. 2020;52:978-993.e6 pubmed publisher
	Deng X, Li S, Sun F. Necrostatin-1 Prevents Necroptosis in Brains after Ischemic Stroke via Inhibition of RIPK1-Mediated RIPK3/MLKL Signaling. Aging Dis. 2019;10:807-817 pubmed publisher
	Taraborrelli L, Peltzer N, Montinaro A, Kupka S, Rieser E, Hartwig T, et al. LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L. Nat Commun. 2018;9:3910 pubmed publisher
	Guo H, Gilley R, Fisher A, Lane R, Landsteiner V, Ragan K, et al. Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1. Cell Death Dis. 2018;9:816 pubmed publisher
	Armaka M, Ospelt C, Pasparakis M, Kollias G. The p55TNFR-IKK2-Ripk3 axis orchestrates arthritis by regulating death and inflammatory pathways in synovial fibroblasts. Nat Commun. 2018;9:618 pubmed publisher
	Ishii T, Okai T, Iwatani Yoshihara M, Mochizuki M, Unno S, Kuno M, et al. CETSA quantitatively verifies in vivo target engagement of novel RIPK1 inhibitors in various biospecimens. Sci Rep. 2017;7:13000 pubmed publisher
	McCabe K, Bacos K, Lu D, Delaney J, Axelrod J, Potter M, et al. Triggering necroptosis in cisplatin and IAP antagonist-resistant ovarian carcinoma. Cell Death Dis. 2014;5:e1496 pubmed publisher
	Cook W, Moujalled D, Ralph T, Lock P, Young S, Murphy J, et al. RIPK1- and RIPK3-induced cell death mode is determined by target availability. Cell Death Differ. 2014;21:1600-12 pubmed publisher
	Moujalled D, Cook W, Murphy J, Vaux D. Necroptosis induced by RIPK3 requires MLKL but not Drp1. Cell Death Dis. 2014;5:e1086 pubmed publisher

product information

Catalog Number :

MABC604

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-MLKL Antibody, clone 3H1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9D2Y4

Host Name :

Rat

Antigen :

Mlkl

Clone :

3H1

Conjugate :

Purified

Purification Method :

Protein G purified

Isotype :

IgG

Product Description :

Anti-MLKL Antibody, clone 3H1

Cross Reactivity :

Mouse

Background :

MLKL, also known as Mixed lineage kinase domain-like protein, and encoded by the gene MLKL, is an interesting protein that is required for the execution of programmed necrosis or necroptosis, for example in response to TNF-alpha induced necrosis. MLKL is a component of the "necrosome," the multiprotein complex that triggers tumor necrosis factor (TNF)-induced cell death by necroptosis. The programmed necrosis induced by TNF-α also requires the activities of the receptor-interacting serine-threonine kinases RIP1 and RIP3 and they too interact directly with the mixed lineage kinase domain-like protein MLKL. RIP3 is an essential upstream kinase in necroptosis. The pseudokinase MLKL functions as a substrate of RIP3 to mediate downstream signaling and when complexed to MLKL the RIP3-MLKL complex is stabilized by AMP-PNP to adopt an inactive conformation.

ALT Names :

Mixed lineage kinase domain-like protein, MLKL

Immunogen :

KLH-conjugated linear peptide corresponding to mouse MLKL.

Package Size :

100 μg

Uses :

Western Blotting, Immunoprecipitation, Immunofluorescence

Storage :

Stable for 1 year at 2-8°C from date of receipt