BRCA1 Antibody, clone MS110 | EMD Millipore MABC199 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BRCA1 Antibody, clone MS110

catalog :

MABC199

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MS110

The same clone is also sold as:

Bio-Rad: MCA1682GT
Abcam: ab16780
Invitrogen: MA1-137

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 5c western blot; human; fig 5a	Kim B, Jeong Y, Wong M, Martin L. DNA damage accumulates and responses are engaged in human ALS brain and spinal motor neurons and DNA repair is activatable in iPSC-derived motor neurons with SOD1 mutations. Acta Neuropathol Commun. 2020;8:7 pubmed publisher
immunocytochemistry; human; fig 2	Vincent A, Berthel E, Dacheux E, Magnard C, Venezia N. BRCA1 affects protein phosphatase 6 signalling through its interaction with ANKRD28. Biochem J. 2016;473:949-60 pubmed publisher
immunohistochemistry - paraffin section; human; 1:75; fig 1	Schirosi L, De Summa S, Tommasi S, Paradiso A, Sambiasi D, Popescu O, et al. Immunoprofile from tissue microarrays to stratify familial breast cancer patients. Oncotarget. 2015;6:27865-79 pubmed publisher
immunohistochemistry - paraffin section; human; 0.1 mg/ml; fig s1	Scalia C, Gendusa R, Cattoretti G. A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection. Appl Immunohistochem Mol Morphol. 2016;24:436-46 pubmed publisher
western blot; human; fig 6	Butin Israeli V, Adam S, Jain N, Otte G, Neems D, WiesmÃ¼ller L, et al. Role of lamin b1 in chromatin instability. Mol Cell Biol. 2015;35:884-98 pubmed publisher
immunohistochemistry - paraffin section; human; 1:150; tbl 2	Green A, Caracappa D, Benhasouna A, Alshareeda A, Nolan C, Macmillan R, et al. Biological and clinical significance of PARP1 protein expression in breast cancer. Breast Cancer Res Treat. 2015;149:353-62 pubmed publisher
immunocytochemistry; human western blot; human	Vidi P, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, et al. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic Acids Res. 2014;42:6365-79 pubmed publisher
	Tokunaga E, Fujita A, Takizawa K, Baba K, Akiyoshi S, Nakamura Y, et al. CD44v9 as a poor prognostic factor of triple-negative breast cancer treated with neoadjuvant chemotherapy. Breast Cancer. 2019;26:47-57 pubmed publisher
	Hodgson D, Dougherty B, Lai Z, Fielding A, Grinsted L, Spencer S, et al. Candidate biomarkers of PARP inhibitor sensitivity in ovarian cancer beyond the BRCA genes. Br J Cancer. 2018;119:1401-1409 pubmed publisher
	Nacson J, Krais J, Bernhardy A, Clausen E, Feng W, Wang Y, et al. BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance. Cell Rep. 2018;24:3513-3527.e7 pubmed publisher
	Seo A, Steinberg Shemer O, Unal S, Casadei S, Walsh T, Gumruk F, et al. Mechanism for survival of homozygous nonsense mutations in the tumor suppressor gene BRCA1. Proc Natl Acad Sci U S A. 2018;115:5241-5246 pubmed publisher
	Patel D, Misenko S, Her J, Bunting S. BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites. J Cell Biol. 2017;216:3521-3534 pubmed publisher
	Liu Y, Cussiol J, Dibitetto D, Sims J, Twayana S, Weiss R, et al. TOPBP1Dpb11 plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1Rad9. J Cell Biol. 2017;216:623-639 pubmed publisher
	Daniels S, Burghel G, Chambers P, Al Baba S, Connley D, Brock I, et al. Levels of DNA Methylation Vary at CpG Sites across the BRCA1 Promoter, and Differ According to Triple Negative and "BRCA-Like" Status, in Both Blood and Tumour DNA. PLoS ONE. 2016;11:e0160174 pubmed publisher
	Wang Y, Krais J, Bernhardy A, Nicolas E, Cai K, Harrell M, et al. RING domain-deficient BRCA1 promotes PARP inhibitor and platinum resistance. J Clin Invest. 2016;126:3145-57 pubmed publisher
	Fleury H, Communal L, Carmona E, Portelance L, Arcand S, Rahimi K, et al. Novel high-grade serous epithelial ovarian cancer cell lines that reflect the molecular diversity of both the sporadic and hereditary disease. Genes Cancer. 2015;6:378-398 pubmed
	Xie J, Peng M, Guillemette S, Quan S, Maniatis S, Wu Y, et al. FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. PLoS Genet. 2012;8:e1002786 pubmed publisher
	Paul I, Savage K, Blayney J, Lamers E, Gately K, Kerr K, et al. PARP inhibition induces BAX/BAK-independent synthetic lethality of BRCA1-deficient non-small cell lung cancer. J Pathol. 2011;224:564-74 pubmed publisher
	Nelson A, Holt J. Impact of RING and BRCT domain mutations on BRCA1 protein stability, localization and recruitment to DNA damage. Radiat Res. 2010;174:1-13 pubmed publisher
	Morris J, Boutell C, Keppler M, Densham R, Weekes D, Alamshah A, et al. The SUMO modification pathway is involved in the BRCA1 response to genotoxic stress. Nature. 2009;462:886-90 pubmed publisher

product information

Catalog Number :

MABC199

Subcategory :

Cell Structure

Product Name :

Anti-BRCA1 Antibody, clone MS110

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P38398

Host Name :

Mouse

Antigen :

BRCA1

Clone :

MS110

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-BRCA1 Antibody, clone MS110

Cross Reactivity :

Human

Background :

Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.

ALT Names :

Breast cancer type 1 susceptibility protein;RING finger protein 53

Immunogen :

Recombinant protein corresponding to the N-terminus of human BRCA1.

Specificity :

This antibody recognizes the N-terminus of BRCA1.

Package Size :

100 μg

Uses :

Western Blotting;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt