This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Collagen Type II Antibody, clone 6B3
catalog :
MAB8887
quantity :
50 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6B3

The same clone is also sold as:
reactivity :
human, mouse, rat, chicken, bovine, domestic sheep
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 59
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 2a
Yano F, Ohba S, Murahashi Y, Tanaka S, Saito T, Chung U. Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation. Sci Rep. 2019;9:7666 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3d
Akbari P, Waldman S, Propst E, Cushing S, Weber J, Yeger H, et al. Generating Mechanically Stable, Pediatric, and Scaffold-Free Nasal Cartilage Constructs In Vitro. Tissue Eng Part C Methods. 2016;22:1077-1084 pubmed
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 2c
Bianchi V, Weber J, Waldman S, Backstein D, Kandel R. Formation of Hyaline Cartilage Tissue by Passaged Human Osteoarthritic Chondrocytes. Tissue Eng Part A. 2017;23:156-165 pubmed publisher
  • immunohistochemistry; human; fig 1
Huang X, ZHu B, Wang X, Xiao R, Wang C. Three-dimensional co-culture of mesenchymal stromal cells and differentiated osteoblasts on human bio-derived bone scaffolds supports active multi-lineage hematopoiesis in vitro: Functional implication of the biomimetic HSC niche. Int J Mol Med. 2016;38:1141-51 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 5c
Abe S, Yamaguchi S, Sato Y, Harada K. Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells. Stem Cells Transl Med. 2016;5:117-28 pubmed publisher
  • western blot; mouse; fig s5c
Kim J, Lee G, Won Y, Lee M, Kwak J, Chun C, et al. Matrix cross-linking-mediated mechanotransduction promotes posttraumatic osteoarthritis. Proc Natl Acad Sci U S A. 2015;112:9424-9 pubmed publisher
  • immunohistochemistry; chicken; 1:500; fig 7e-h
Schock E, Chang C, Struve J, Chang Y, Chang J, Delany M, et al. Using the avian mutant talpid2 as a disease model for understanding the oral-facial phenotypes of oral-facial-digital syndrome. Dis Model Mech. 2015;8:855-66 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Li W, Cai L, Zhang Y, Cui L, Shen G. Intra-articular resveratrol injection prevents osteoarthritis progression in a mouse model by activating SIRT1 and thereby silencing HIF-2α. J Orthop Res. 2015;33:1061-70 pubmed publisher
  • immunohistochemistry - paraffin section; domestic sheep; 1:200
Caminal M, Peris D, Fonseca C, Barrachina J, Codina D, Rabanal R, et al. Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect. Cytotechnology. 2016;68:907-19 pubmed publisher
  • immunohistochemistry - paraffin section; human
Xiao L, Kumazawa Y, Okamura H. Cell death, cavitation and spontaneous multi-differentiation of dental pulp stem cells-derived spheroids in vitro: a journey to survival and organogenesis. Biol Cell. 2014;106:405-19 pubmed publisher
  • immunocytochemistry; rat; 1:100
Pokrywczynska M, Jundzill A, Adamowicz J, Kowalczyk T, Warda K, Rasmus M, et al. Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?. PLoS ONE. 2014;9:e105295 pubmed publisher
  • immunohistochemistry - paraffin section; bovine; 1:100
Ahmed N, Iu J, Brown C, Taylor D, Kandel R. Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction. Tissue Eng Part A. 2014;20:2224-33 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:300
Zhang J, Wang J. Prostaglandin E2 (PGE2) exerts biphasic effects on human tendon stem cells. PLoS ONE. 2014;9:e87706 pubmed publisher
  • western blot; mouse; fig 3a
Shin Y, Huh Y, Kim K, Kim S, Park K, Koh J, et al. Low-density lipoprotein receptor-related protein 5 governs Wnt-mediated osteoarthritic cartilage destruction. Arthritis Res Ther. 2014;16:R37 pubmed publisher
  • immunocytochemistry; human
Bouacida A, Rosset P, Trichet V, Guilloton F, Espagnolle N, Cordonier T, et al. Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells. PLoS ONE. 2012;7:e48648 pubmed publisher
  • immunocytochemistry; human
Jager M, Thorey F, Westhoff B, Wild A, Krauspe R. In vitro osteogenic differentiation is affected in Wiedemann-Rautenstrauch-Syndrome (WRS). In Vivo. 2005;19:831-6 pubmed
Wang L, Yang M, Zhang C, Huang F. The protective effects of dehydrocostus lactone against TNF-α-induced degeneration of extracellular matrix (ECM) in SW1353 cells. Aging (Albany NY). 2020;12:17137-17149 pubmed publisher
Xu Z, Ke T, Zhang Y, Fu C, He W. Agonism of GPR120 prevented IL-1β-induced reduction of extracellular matrix through SOX-9. Aging (Albany NY). 2020;12:12074-12085 pubmed publisher
Murahashi Y, Yano F, Kobayashi H, Makii Y, Iba K, Yamashita T, et al. Intra-articular administration of IκBα kinase inhibitor suppresses mouse knee osteoarthritis via downregulation of the NF-κB/HIF-2α axis. Sci Rep. 2018;8:16475 pubmed publisher
Wang Q, Tan Q, Xu W, Kuang L, Zhang B, Wang Z, et al. Postnatal deletion of Alk5 gene in meniscal cartilage accelerates age-dependent meniscal degeneration in mice. J Cell Physiol. 2018;234:595-605 pubmed publisher
Golding A, Guay J, Herrera Rincon C, Levin M, Kaplan D. A Tunable Silk Hydrogel Device for Studying Limb Regeneration in Adult Xenopus Laevis. PLoS ONE. 2016;11:e0155618 pubmed publisher
Ding Z, Huang H. Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair. BMC Musculoskelet Disord. 2015;16:65 pubmed publisher
Taylor D, Ahmed N, Parreno J, Lunstrum G, Gross A, Diamandis E, et al. Collagen type XII and versican are present in the early stages of cartilage tissue formation by both redifferentating passaged and primary chondrocytes. Tissue Eng Part A. 2015;21:683-93 pubmed publisher
Chen J, Kamiya Y, Polur I, Xu M, Choi T, Kalajzic Z, et al. Estrogen via estrogen receptor beta partially inhibits mandibular condylar cartilage growth. Osteoarthritis Cartilage. 2014;22:1861-8 pubmed publisher
Peck Y, Ng L, Goh J, Gao C, Wang D. A three-dimensionally engineered biomimetic cartilaginous tissue model for osteoarthritic drug evaluation. Mol Pharm. 2014;11:1997-2008 pubmed publisher
Khattak S, Schuez M, Richter T, Knapp D, Haigo S, Sandoval Guzmán T, et al. Germline transgenic methods for tracking cells and testing gene function during regeneration in the axolotl. Stem Cell Reports. 2013;1:90-103 pubmed publisher
Pelosi M, Lazzarano S, Thoms B, Murphy C. Parathyroid hormone-related protein is induced by hypoxia and promotes expression of the differentiated phenotype of human articular chondrocytes. Clin Sci (Lond). 2013;125:461-70 pubmed publisher
Schüettler K, Struewer J, Rominger M, Rexin P, Efe T. Repair of a chondral defect using a cell free scaffold in a young patient--a case report of successful scaffold transformation and colonisation. BMC Surg. 2013;13:11 pubmed publisher
Magi G, Renzoni G, Piccionello A, Rossi G. Primary ocular chondrosarcoma in a discus (Symphisodon aequifasciatus). J Zoo Wildl Med. 2013;44:225-31 pubmed
Saw K, Anz A, Siew Yoke Jee C, Merican S, Ching Soong Ng R, Roohi S, et al. Articular cartilage regeneration with autologous peripheral blood stem cells versus hyaluronic acid: a randomized controlled trial. Arthroscopy. 2013;29:684-94 pubmed publisher
Kamiya Y, Chen J, Xu M, Utreja A, Choi T, Drissi H, et al. Increased mandibular condylar growth in mice with estrogen receptor beta deficiency. J Bone Miner Res. 2013;28:1127-34 pubmed publisher
Leong W, Lau T, Wang D. A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system. Acta Biomater. 2013;9:6459-67 pubmed publisher
Cavallo C, Desando G, Columbaro M, Ferrari A, Zini N, Facchini A, et al. Chondrogenic differentiation of bone marrow concentrate grown onto a hylauronan scaffold: rationale for its use in the treatment of cartilage lesions. J Biomed Mater Res A. 2013;101:1559-70 pubmed publisher
Cosden Decker R, Bickett M, Lattermann C, MacLeod J. Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders. Osteoarthritis Cartilage. 2012;20:1347-56 pubmed publisher
Sternberg H, Murai J, Erickson I, Funk W, Das S, Wang Q, et al. A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 2012;7:481-501 pubmed publisher
Yamachika E, Tsujigiwa H, Matsubara M, Hirata Y, Kita K, Takabatake K, et al. Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells (MSCs) and regeneration of bone from MSC in vivo. J Mol Histol. 2012;43:223-33 pubmed publisher
Li X, Chu J, Wang A, Zhu Y, Chu W, Yang L, et al. Uniaxial mechanical strain modulates the differentiation of neural crest stem cells into smooth muscle lineage on micropatterned surfaces. PLoS ONE. 2011;6:e26029 pubmed publisher
Saw K, Anz A, Merican S, Tay Y, Ragavanaidu K, Jee C, et al. Articular cartilage regeneration with autologous peripheral blood progenitor cells and hyaluronic acid after arthroscopic subchondral drilling: a report of 5 cases with histology. Arthroscopy. 2011;27:493-506 pubmed publisher
Cosden R, Lattermann C, Romine S, Gao J, Voss S, MacLeod J. Intrinsic repair of full-thickness articular cartilage defects in the axolotl salamander. Osteoarthritis Cartilage. 2011;19:200-5 pubmed publisher
Gong Y, Su K, Lau T, Zhou R, Wang D. Microcavitary hydrogel-mediating phase transfer cell culture for cartilage tissue engineering. Tissue Eng Part A. 2010;16:3611-22 pubmed publisher
Varshney R, Zhou R, Hao J, Yeo S, Chooi W, Fan J, et al. Chondrogenesis of synovium-derived mesenchymal stem cells in gene-transferred co-culture system. Biomaterials. 2010;31:6876-91 pubmed publisher
Fan J, Ren L, Liang R, Gong Y, Cai D, Wang D. Chondrogenesis of synovium-derived mesenchymal stem cells in photopolymerizing hydrogel scaffolds. J Biomater Sci Polym Ed. 2010;21:1653-67 pubmed publisher
Dudek K, Lafont J, Martinez Sanchez A, Murphy C. Type II collagen expression is regulated by tissue-specific miR-675 in human articular chondrocytes. J Biol Chem. 2010;285:24381-7 pubmed publisher
Ramos C, Asgari Z, Liu E, Yvon E, Heslop H, Rooney C, et al. An inducible caspase 9 suicide gene to improve the safety of mesenchymal stromal cell therapies. Stem Cells. 2010;28:1107-15 pubmed publisher
Thoms B, Murphy C. Inhibition of hypoxia-inducible factor-targeting prolyl hydroxylase domain-containing protein 2 (PHD2) enhances matrix synthesis by human chondrocytes. J Biol Chem. 2010;285:20472-80 pubmed publisher
Toh W, Lee E, Richards M, Cao T. In vitro derivation of chondrogenic cells from human embryonic stem cells. Methods Mol Biol. 2010;584:317-31 pubmed publisher
Fan J, Gong Y, Ren L, Varshney R, Cai D, Wang D. In vitro engineered cartilage using synovium-derived mesenchymal stem cells with injectable gellan hydrogels. Acta Biomater. 2010;6:1178-85 pubmed publisher
Mehlhorn A, Zwingmann J, Finkenzeller G, Niemeyer P, Dauner M, Stark B, et al. Chondrogenesis of adipose-derived adult stem cells in a poly-lactide-co-glycolide scaffold. Tissue Eng Part A. 2009;15:1159-67 pubmed publisher
Gat Yablonski G, Shtaif B, Abraham E, Phillip M. Nutrition-induced catch-up growth at the growth plate. J Pediatr Endocrinol Metab. 2008;21:879-93 pubmed
Sotiropoulou P, Perez S, Papamichail M. Clinical grade expansion of human bone marrow mesenchymal stem cells. Methods Mol Biol. 2007;407:245-63 pubmed publisher
Blair Levy J, Watts C, Fiorentino N, Fiorientino N, Dimitriadis E, Marini J, et al. A type I collagen defect leads to rapidly progressive osteoarthritis in a mouse model. Arthritis Rheum. 2008;58:1096-106 pubmed publisher
Olivotto E, Borzi R, Vitellozzi R, Pagani S, Facchini A, Battistelli M, et al. Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes. Arthritis Rheum. 2008;58:227-39 pubmed publisher
Lafont J, Talma S, Murphy C. Hypoxia-inducible factor 2alpha is essential for hypoxic induction of the human articular chondrocyte phenotype. Arthritis Rheum. 2007;56:3297-306 pubmed
Butz D, Li G, Huebner S, Cook M. A mechanistic approach to understanding conjugated linoleic acid's role in inflammation using murine models of rheumatoid arthritis. Am J Physiol Regul Integr Comp Physiol. 2007;293:R669-76 pubmed
Nöth U, Rackwitz L, Heymer A, Weber M, Baumann B, Steinert A, et al. Chondrogenic differentiation of human mesenchymal stem cells in collagen type I hydrogels. J Biomed Mater Res A. 2007;83:626-35 pubmed
Ryu J, Chun J. Opposing roles of WNT-5A and WNT-11 in interleukin-1beta regulation of type II collagen expression in articular chondrocytes. J Biol Chem. 2006;281:22039-47 pubmed publisher
Hwang S, Yu S, Poo H, Chun J. c-Jun/activator protein-1 mediates interleukin-1beta-induced dedifferentiation but not cyclooxygenase-2 expression in articular chondrocytes. J Biol Chem. 2005;280:29780-7 pubmed
zur Nieden N, Kempka G, Rancourt D, Ahr H. Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: effect of cofactors on differentiating lineages. BMC Dev Biol. 2005;5:1 pubmed
Grigolo B, Roseti L, De Franceschi L, Piacentini A, Cattini L, Manfredini M, et al. Molecular and immunohistological characterization of human cartilage two years following autologous cell transplantation. J Bone Joint Surg Am. 2005;87:46-57 pubmed
product information
Catalog Number :
MAB8887
Subcategory :
Cell Structure
Product Name :
Anti-Collagen Type II Antibody, clone 6B3
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P02458
Host Name :
Mouse
Antigen :
Collagen Type II
Clone :
6B3
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Collagen Type II Antibody, clone 6B3
Cross Reactivity :
Chicken;Human;Mouse;Salamander
Immunogen :
Purified type II collagen; obtained from adult chicken sternum by limited pepsinization.
Specificity :
In Western blotting (but not ELISA), MAB8887 recognizes both a1(II) and a3(XI) chains which have identical primary structure. In Western blotting, MAB8887 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. It will not react with intact Collagen II under traditional conditions. Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.
Package Size :
50 μg
Uses :
Immunofluorescence;Immunohistochemistry;Western Blotting
Storage :
Maintain at 2–8°C in undiluted aliquots for up to 12 months
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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